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Human SOX2 Biotinylated Antibody

Catalog #: BAF2018
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
BAF2018
SOX2 in Human iPSK3 cells.
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Citations (2)
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Human SOX2 Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects SOX2 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human (rh) SOX17 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human SOX2
Gly135-Met317
Accession # P48431
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human SOX2
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry SOX2 antibody in Human iPSK3 cells by Immunocytochemistry (ICC). View Larger

SOX2 in Human iPSK3 cells. SOX2 was detected in immersion fixed human plasmid-derived induced pluripotent stem cells (iPSK3) using Goat Anti-Human SOX2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF2018) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SOX2

SOX2 belongs to the SOX (SRY-like HMG box) family of transcription factors with diverse roles in development. SOX2 functions in specifying the first three lineages present at implantation and in regulating proliferation and differentiation in the developing peripheral nervous system (1-6).

References
  1. Graham, V. et al. (2003) Neuron 39:749.
  2. Avilion, A.A. et al. (2003) Genes Dev. 17:126.
  3. Kishi, M. et al. (2000) Development 127:791.
  4. Yuan, H. et al. (1995) Genes Dev. 9:2635.
  5. Uwanogho, D. et al. (1995) Mech. Dev. 49:23.
  6. Stevanovic, M. (2003) Mol. Biol. Rep. 30:127.
Long Name
Transcription Factor SOX2
Entrez Gene IDs
6657 (Human)
Alternate Names
ANOP3; MCOPS3; MGC2413; SOX2; SRY (sex determining region Y)-box 2; SRY-related HMG-box gene 2; transcription factor SOX2; transcription factor SOX-2

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Citations for Human SOX2 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Eva1 Maintains the Stem-like Character of Glioblastoma-Initiating Cells by Activating the Noncanonical NF-?B Signaling Pathway
    Authors: N Ohtsu, Y Nakatani, D Yamashita, S Ohue, T Ohnishi, T Kondo
    Cancer Res., 2015-12-17;76(1):171-81.
    Species: Human, Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC
  2. Conversion of differentiated cancer cells into cancer stem-like cells in a glioblastoma model after primary chemotherapy.
    Authors: Auffinger B, Tobias A, Han Y, Lee G, Guo D, Dey M, Lesniak M, Ahmed A
    Cell Death Differ, 2014-03-07;21(7):1119-31.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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