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Human SOX17 Antibody

Catalog #: MAB1924
56 Citations 1 Review Datasheet / COA / SDS
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MAB1924
MAB1924-SP
SOX17 in B16 Mouse Cell Line.
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Citations (56)
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Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human SOX17 Antibody Summary

Species Reactivity
Human
Specificity
Detects human SOX17 in direct ELISAs. In direct ELISAs, this antibody does not cross-react with recombinant human SOX2, 3, 7, or 10.
Source
Monoclonal Mouse IgG3 Clone # 245013
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human SOX17
Asp177-Val414
Accession # Q9H6I2
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry SOX17 antibody in B16 Mouse Cell Line by Immunocytochemistry (ICC). View Larger

SOX17 in B16 Mouse Cell Line. SOX17 was detected in immersion fixed B16 mouse melanoma cell line using Mouse Anti-Human SOX17 Monoclonal Antibody (Catalog # MAB1924) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SOX17

SOX17 is a member of the SOX family of transcription factors that bind DNA via a high mobility group (HMG) domain. SOX17 is suggested to play an important role in endoderm development (1, 2).

References
  1. Kanai-Azuma, M. et al. (2002) Development 129:2367.
  2. Katoh, M. et al. (2002) Int. J. Mol. Med. 9:153.
Long Name
Transcription Factor SOX17
Entrez Gene IDs
64321 (Human)
Alternate Names
FLJ22252; SOX17; SRY (sex determining region Y)-box 17; SRY-related HMG-box transcription factor SOX17; transcription factor SOX-17; VUR3

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Citations for Human SOX17 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

56 Citations: Showing 1 - 10
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  1. Interplay of Oct4 with Sox2 and Sox17: a molecular switch from stem cell pluripotency to specifying a cardiac fate
    Authors: Sonia Stefanovic, Nesrine Abboud, Stéphanie Désilets, David Nury, Chad Cowan, Michel Pucéat
    Journal of Cell Biology
  2. Differentiation of immortalized human multi-lineage progenitor to alveolar type 2-like cells: angiotensin-converting enzyme 2 expression and binding of severe acute respiratory syndrome coronavirus 2 spike and spike 1 proteins
    Authors: Collins DP, Osborn MJ, Steer CJ. et al.
    Cytotherapy
  3. Generation and characterization of integration-free induced pluripotent stem cells from patients with autoimmune disease
    Authors: Mi-Young Son, Mi-Ok Lee, Hyejin Jeon, Binna Seol, Jung Hwa Kim, Jae-Suk Chang et al.
    Experimental & Molecular Medicine
  4. Transplantation of Human Embryonic Stem Cell-Derived Pancreatic Endoderm Reveals a Site-Specific Survival, Growth, and Differentiation
    Authors: Lina Sui, Josué K. Mfopou, Bing Chen, Karen Sermon, Luc Bouwens
    Cell Transplantation
  5. Transcriptomic analysis of feeder-free culture system for maintaining naïve-state pluripotency in human pluripotent stem cells
    Authors: Wataru Isono, Tomoyuki Kawasaki, Justin K. Ichida, Kazunori Nagasaka, Osamu Hiraike, Akihiro Umezawa et al.
    Stem Cell Investigation
  6. In Vitro Modeling of Alcohol-Induced Liver Injury Using Human-Induced Pluripotent Stem Cells
    Authors: Lipeng Tian, Neha Prasad, Yoon-Young Jang
    Methods in Molecular Biology
  7. Indolactam V/GLP-1-mediated differentiation of human iPS cells into glucose-responsive insulin-secreting progeny
    Authors: Tayaramma Thatava, Timothy J. Nelson, Ramakrishna Edukulla, Toshie Sakuma, Seiga Ohmine, Jason M. Tonne et al.
    Gene Therapy
  8. Generation of induced pluripotent stem cell lines IRMBi003-A and IRMBi003-B from a healthy donor to model Alzheimer's disease
    Authors: Clua Provost, C;Auboyer, L;Rovelet-Lecrux, A;Monzo, C;Schob, E;Andreux, F;Quittet, C;Lehmann, S;Wallon, D;Crozet, C;
    Stem cell research
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC/IF
  9. Establishment of induced pluripotent stem cells IRMBi005-A from a patient with sporadic Alzheimer's disease
    Authors: Provost, CC;Auboyer, L;Rovelet-Lecrux, A;Monzo, C;Schob, E;Lehmann, S;Wallon, D;Crozet, C;
    Stem cell research
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  10. Generation of an induced pluripotent stem cell line XJHi001-A from a fibronectin glomerulopathy patient carrying a heterozygous NM_212482.2(C.5888-1G > C) mutation in the FN1 gene
    Authors: She, L;Li, X;Bai, M;
    Stem cell research
    Species: Human
    Sample Types: Transfected Whole Cells
    Applications: ICC
  11. Large-scale production of human blastoids amenable to modeling blastocyst development and maternal-fetal cross talk
    Authors: Yu, L;Logsdon, D;Pinzon-Arteaga, CA;Duan, J;Ezashi, T;Wei, Y;Ribeiro Orsi, AE;Oura, S;Liu, L;Wang, L;Liu, K;Ding, X;Zhan, L;Zhang, J;Nahar, A;Stobbe, C;Katz-Jaffe, M;Schoolcraft, WB;Tan, T;Hon, GC;Yuan, Y;Wu, J;
    Cell stem cell
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  12. Generation of an induced pluripotent stem cell line (ZSZOCi001-A) from a patient with Knobloch syndrome caused by biallelic mutations in the gene COL18A1
    Authors: Jiang, Z;Sun, W;Zhang, Q;Wang, P;
    Stem cell research
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  13. Generation of a human fibroblast-derived induced pluripotent stem cell line KRIBBi009-A from a patient with breast cancer
    Authors: N Son, YS Son, CR Jung, MY Son
    Stem Cell Research, 2023-03-14;68(0):103060.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, ICC
  14. Efficient reprogramming of human fibroblasts using RNA reprogramming with DAPT and iDOT1L under normoxia conditions
    Authors: K Morita, A Nakamura, M Machida, T Kawasaki, R Nakanishi, J Ichida, T Iwata, A Umezawa, H Akutsu
    Regenerative Therapy, 2022-09-19;21(0):389-397.
    Species: Human
    Sample Types: Organoid
    Applications: IHC
  15. Generation of a human induced pluripotent stem cell line (LZUSHi002-A) from a MADD patient with ETFDH mutation
    Authors: Y Li, Y Li, H Liu, L Cheng, H Ma, X Xu, N Zhang
    Stem Cell Research, 2022-09-14;64(0):102914.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC
  16. Generation of a homozygous knock-in human embryonic stem cell line expressing mEos4b-tagged CTR1
    Authors: YH Chen, PS Huang, MH Wen, M Pan, DF Lee, TY Chen
    Oncogene, 2022-06-14;63(0):102845.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  17. Generation of an integration-free induced pluripotent stem cell line (LZUSHI001-A) from an epileptic patient with DGKG mutation
    Authors: YQ Liu, TW Ling, HY Wang, YH Yang, WJ Song, TC Wang
    Stem Cell Research, 2022-03-28;61(0):102768.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  18. Generation of a gene corrected human isogenic iPSC line (CPGHi001-A-1) from a hearing loss patient with the TMC1 p.M418K mutation using CRISPR/Cas9
    Authors: H Wang, Y Luo, J Li, J Guan, S Yang, Q Wang
    Stem Cell Research, 2022-02-28;60(0):102736.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  19. Generation of an induced pluripotent stem cell line (FDCHI007-A) derived from a patient with developmental and epileptic encephalopathy Type 31 carrying heterozygous c.545C�>�A mutation in DNM1 gene
    Authors: Y Xu, X Wang, T Qiu, J Wang, Y Wang, B Wu, Y Zhou
    Stem Cell Research, 2022-02-12;60(0):102709.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  20. SOX17 and PAX8 constitute an actionable lineage-survival transcriptional complex in ovarian cancer
    Authors: L Lin, K Shi, S Zhou, MC Cai, C Zhang, Y Sun, J Zang, L Cheng, K Ye, P Ma, P Shen, M Zhang, Y Cheng, C Qi, Y Li, X Yin, Y Zheng, L Tan, G Zhuang, R Zang
    Oncogene, 2022-02-05;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  21. Generation of human induced pluripotent stem cell line, KRIBBi003-A, from urinary cells of a patient with glycogen storage disease type IXa
    Authors: Y Shin, SJ Mun, J Lee, KS Chung, M Kim, C Kun Cheon, MJ Son
    Stem Cell Research, 2021-10-22;57(0):102584.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, ICC
  22. Generation of an induced pluripotent stem cell line (FDCHi006-A) from a 7-year-old girl with central precocious puberty
    Authors: T Fan, J He, Y Wang, J Yu, W Sun
    Stem Cell Research, 2021-09-28;56(0):102542.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, ICC
  23. Generation of insulin-producing pancreatic beta cells from multiple human stem cell lines
    Authors: NJ Hogrebe, KG Maxwell, P Augsornwor, JR Millman
    Nature Protocols, 2021-08-04;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, ICC
  24. Generation of human induced pluripotent stem cell lines from 2 patients with MIRAGE syndrome
    Authors: I Fischer, J Küchler, C Schaar, T Fisch, J Cernoch, K Fischer, V Fernández, P Kühnen, H Stachelsch
    Stem Cell Research, 2021-06-10;54(0):102417.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  25. Generation of an iPSC line (GWCMCi002-A) from an X-linked Alport syndrome patient with a hemizygous splicing mutation (NM_000495.4, c. 1517-1 G�>�T) in the COL4A5 gene
    Authors: X Gao, G Li, H Deng, W Feng, Y Li, M Liu, Y Li
    Stem Cell Research, 2021-05-11;53(0):102388.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  26. Generation of a human induced pluripotent stem cell line (CPGHi003-A) from an auditory neuropathy patient with AIFM1 p.R422Q mutation
    Authors: J Li, K Wu, J Guan, Q Wang, H Wang
    Stem Cell Research, 2021-04-29;53(0):102376.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  27. Generation of an induced pluripotent stem cell line (OGHFUi001-A) from a type 1 early infantile epileptic encephalopathy with ARX mutation
    Authors: N Jia, X Gong, J Chen, T Yang, C Bao, J Shen, X Xiao
    Stem Cell Research, 2021-04-27;53(0):102367.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  28. An induced pluripotent stem cell line (GZHMCi004-A) derived from a fetus with heterozygous G380R mutation in FGFR3 gene causing achondroplasia
    Authors: N Li, SM Lin, Y Li, J Sun, L Zhang, M Chen
    Stem Cell Research, 2021-04-05;53(0):102322.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, ICC
  29. Generation of a gene corrected human isogenic iPSC line (CPGHi002-A-1) from a DDOD patient with heterozygous c.1516 C>T mutation in the ATP6V1B2 gene
    Authors: X Gao, SW Qiu, WQ Wang, DY Kang, N Su, P Dai, YY Yuan
    Stem Cell Research, 2021-02-25;53(0):102271.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  30. Generation of a gene-corrected isogenic iPSC line (AHQUi001-A-1) from a patient with familial hypertriglyceridemia (FHTG) carrying a heterozygous p.C310R (c.928�T�>�C) mutation in LPL gene using CRISPR/Cas9
    Authors: X Sun, X Zhou, B Dong, C Wang, X Xiao, Y Wang
    Stem Cell Research, 2021-02-11;52(0):102230.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, ICC
  31. An induced pluripotent stem cell line (GZHMCi003-A) derived from a fetus with exon 3 heterozygous deletion in RUNX2 gene causing cleidocranial dysplasia
    Authors: M Chen, SM Lin, N Li, Y Li, Y Li, L Zhang
    Stem Cell Research, 2021-01-09;51(0):102166.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, ICC
  32. Derivation of an induced pluripotent stem cell line (GWCMCi001-A) from PBMCs of a four-year-old male patient with Immunoglobulin A nephropathy
    Authors: X Gao, X Liao, J Zhang, J Lin, M Tan
    Stem Cell Research, 2020-12-10;50(0):102123.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  33. Generation of an induced pluripotent stem cell line (PUMCHi006-A) derived from a patient with pulmonary arterial hypertension carrying heterozygous c.1339 G�>�A mutation in PTGIS gene
    Authors: YJ Zhu, SJ Zhang, XH Wu, TY Lian, YZ He, ZJ Zhang, D Lu, K Sun, HF Wang, ZC Jing
    Stem Cell Res, 2020-11-15;49(0):102088.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  34. Generation of gene-corrected iPSCs line (KEIUi001-A) from a PARK8 patient iPSCs with familial Parkinson's disease carrying the I2020T mutation in LRRK2
    Authors: E Ohta, T Sone, H Ukai, T Hisamatsu, T Kitagawa, M Ishikawa, M Nagai, HR Ueda, F Obata, H Okano
    Stem Cell Res, 2020-11-03;49(0):102073.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  35. Establishment of human induced pluripotent stem cell line (CPGHi002-A) from a 10-month-old female patient with DDOD syndrome carrying a heterozygous c.1516 C�>�T mutation in ATP6V1B2
    Authors: X Gao, SW Qiu, ML Feng, SS Huang, DY Kang, MY Han, P Dai, YY Yuan
    Stem Cell Res, 2020-09-08;48(0):101986.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  36. The combination of dibenzazepine and a DOT1L inhibitor enables a stable maintenance of human na�ve-state pluripotency in non-hypoxic conditions
    Authors: W Isono, T Kawasaki, JK Ichida, T Ayabe, O Hiraike, A Umezawa, H Akutsu
    Regenerative Therapy, 2020-09-02;15(0):161-168.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  37. Generation of an induced pluripotent stem cell line (CHFUi001-A) from an osteogenesis imperfecta patient with COL1A2 mutation
    Authors: Z Zheng, W Lu, Z Pei, J Chen, T Yang, F Luo
    Stem Cell Res, 2020-07-05;47(0):101907.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  38. An induced pluripotent stem cell line (SHEHi002-A (5426))from a patient of long QT syndrome type 8 with c.2573G>A mutation in the gene CACNA1C
    Authors: HM Zhou, XQ Zhou, JZ Lu, WW Jia, JH Kang
    Stem Cell Res, 2020-02-06;43(0):101731.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  39. Measles vector as a multiple genes delivery platform facilitating iPSC reprogramming
    Authors: Q Wang, A Vossen, Y Ikeda, P Devaux
    Gene Ther., 2019-02-04;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  40. Generation of two human isogenic iPSC lines from fetal dermal fibroblasts
    Authors: R Tandon, B Brändl, N Baryshniko, A Landshamme, L Steenpa beta, O Keminer, O Pless, FJ Müller
    Stem Cell Res, 2018-10-12;33(0):120-124.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  41. Low Cell-Matrix Adhesion Reveals Two Subtypes of Human Pluripotent Stem Cells
    Authors: L Yu, J Li, J Hong, Y Takashima, N Fujimoto, M Nakajima, A Yamamoto, X Dong, Y Dang, Y Hou, W Yang, I Minami, K Okita, M Tanaka, C Luo, F Tang, Y Chen, C Tang, H Kotera, L Liu
    Stem Cell Reports, 2018-06-28;11(1):142-156.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  42. Generation of an induced pluripotent stem cell line from a patient with adult-onset leukoencephalopathy with axonal spheroids and pigmented glia (ALSP): HIHCNi003-A
    Authors: SN Hayer, Y Schelling, P Hoeflinger, S Hauser, L Schöls
    Stem Cell Res, 2018-06-20;30(0):206-209.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  43. PI3K/mTORC2 regulates TGF-beta/Activin signalling by modulating Smad2/3 activity via linker phosphorylation.
    Authors: Yu J, Ramasamy T, Murphy N, Holt M, Czapiewski R, Wei S, Cui W
    Nat Commun, 2015-05-22;6(0):7212.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: IHC, Western Blot
  44. Intrapatient variations in type 1 diabetes-specific iPS cell differentiation into insulin-producing cells.
    Authors: Thatava T, Kudva Y, Edukulla R, Squillace K, De Lamo J, Khan Y, Sakuma T, Ohmine S, Terzic A, Ikeda Y
    Mol Ther, 2012-11-27;21(1):228-39.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, IHC-Fr
  45. FGF signaling via MAPK is required early and improves Activin A-induced definitive endoderm formation from human embryonic stem cells.
    Authors: Sui L, Mfopou J, Geens M, Sermon K, Bouwens L
    Biochem Biophys Res Commun, 2012-08-29;426(3):380-5.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  46. New cell lines from mouse epiblast share defining features with human embryonic stem cells.
    Authors: Tesar PJ, Chenoweth JG, Brook FA, Davies TJ, Evans EP, Mack DL, Gardner RL, McKay RD
    Nature, 2007-06-27;448(7150):196-9.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  47. Conditional gene expression in human embryonic stem cells.
    Authors: Vallier L, Alexander M, Pedersen R
    Stem Cells, 2007-02-22;25(6):1490-7.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  48. Neurogenic effect of vascular endothelial growth factor during germ layer formation of human embryonic stem cells.
    Authors: Kim BK, Kim SE, Shim JH, Woo DH, Gil JE, Kim SK, Kim JH
    FEBS Lett., 2006-10-02;580(25):5869-74.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  49. Reprogrammed keratinocytes from elderly type 2 diabetes patients suppress senescence genes to acquire induced pluripotency
    Authors: Seiga Ohmine, Karen A. Squillace, Katherine A. Hartjes, Michael C. Deeds, Adam S. Armstrong, Tayaramma Thatava et al.
    Aging (Albany NY)

  50. Hepatic Differentiation of Marmoset Embryonic Stem Cells and Functional Characterization of ESC-Derived Hepatocyte-Like Cells
    Authors: Rajagopal N Aravalli, Daniel P Collins, Joel H Hapke, Andrew T Crane, Clifford J Steer
    Hepatic Medicine: Evidence and Research
  51. Engineering human hepato-biliary-pancreatic organoids from pluripotent stem cells
    Authors: Hiroyuki Koike, Kentaro Iwasawa, Rie Ouchi, Mari Maezawa, Masaki Kimura, Asuka Kodaka et al.
    Nature Protocols
  52. Induced pluripotent stem cells from GMP-grade hematopoietic progenitor cells and mononuclear myeloid cells
    Authors: Seiga Ohmine, Allan B Dietz, Michael C Deeds, Katherine A Hartjes, David R Miller, Tayaramma Thatava et al.
    Stem Cell Research & Therapy
  53. Effects of the Extracts from Fruit and Stem of Camellia japonica on Induced Pluripotency and Wound Healing
    Authors: H Jeon, JY Kim, JK Choi, E Han, CL Song, J Lee, YS Cho
    J Clin Med, 2018-11-20;7(11):.
  54. Changes in the hepatic differentiation potential of human mesenchymal stem cells aged in vitro
    Authors: Sang Luo, Shuai Xiao, Yang Ai, Ben Wang, Yefu Wang
    Annals of Translational Medicine
  55. Early gestation chorionic villi-derived stromal cells for fetal tissue engineering
    Authors: Lee Lankford, Taryn Selby, James Becker, Volodymyr Ryzhuk, Connor Long, Diana Farmer et al.
    World Journal of Stem Cells
  56. Intrinsic Immunity Shapes Viral Resistance of Stem Cells
    Authors: Xianfang Wu, Viet Loan Dao Dao Thi, Yumin Huang, Eva Billerbeck, Debjani Saha, Hans-Heinrich Hoffmann et al.
    Cell

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Human SOX17 Antibody
By Anonymous on 08/23/2021
Application: Immunocytochemistry/Immunofluorescence Sample Tested: iPS2 human induced pluripotent stem cells Species: Human

It worked well in 1:200 dilution

Immunocytochemistry/Immunofluorescence Human SOX17 Antibody MAB1924