Human SMAC/Diablo Biotinylated Antibody

Catalog #: BAF789 Datasheet / COA / SDS

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BAF789

All SMAC/Diablo Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human SMAC/Diablo Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human SMAC in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human SMAC
Ala56-Asp239
Accession # Q9NR28

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human SMAC/Diablo (Catalog # 789-SM)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SMAC/Diablo

SMAC (second mitochondria derived activator of caspase)/Diablo promotes caspase activation by interacting with the inhibitor of apoptosis (IAP) proteins in the cytochrome c/Apaf-1/caspase-9 pathway.

References

Du, C. et al. (2000) Cell 102:33.
Chai, J. et al. (2000) Nature 406:855.
Srinivasula, S. et al. (2000) J. Biol. Chem. 275:36152.
Ekert, P. et al. (2001) J. Cell Biol. 152:483.
Verhagen, A. (2000) Cell 102:43.

Long Name

Second Mitochondria-derived Activator of Caspase

Entrez Gene IDs

56616 (Human)

Alternate Names

0610041G12Rik; Diablo; diablo, IAP-binding mitochondrial protein; Direct IAP-binding protein with low pI; mitochondrial Smac protein; Second mitochondria-derived activator of caspase; SMAC; SMACmitochondrial