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Human SMAC/Diablo Antibody

Catalog #: AF789
7 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF789
AF789-SP
Detection of Human SMAC/Diablo by Western Blot.
2 Images
Product Details
Citations (7)
FAQs
Supplemental Products
Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human SMAC/Diablo Antibody Summary

Species Reactivity
Human
Specificity
Detects human SMAC/Diablo in Western blots. Also detects mouse and rat SMAC/Diablo when the salt concentration of blotting buffer is reduced to 0.15 M NaCl, and 1 μg/mL anti-SMAC/Diablo concentration is used.
Source
Polyclonal Rabbit IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human SMAC/Diablo
Ala56-Asp239
Accession # Q9NR28
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
Simple Western
5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human SMAC/Diablo antibody by Western Blot. View Larger

Detection of Human SMAC/Diablo by Western Blot. Western blot shows lysates of SH-SY5Y human neuroblastoma cell line, CHP-100 human neuroblastoma cell line, and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 0.5 µg/mL Rabbit Anti-Human SMAC/Diablo Antigen Affinity-purified Polyclonal Antibody (Catalog # AF789) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). For additional reference, Recombinant Human SMAC/Diablo (Catalog # 789-SM) (0.5 ng/lane) was included. A specific band for SMAC/Diablo was detected at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 6.

Simple Western Detection of Human SMAC/Diablo antibody by Simple Western<SUP>TM</SUP>. View Larger

Detection of Human SMAC/Diablo by Simple WesternTM. Simple Western lane view shows lysates of SH‑SY5Y human neuroblastoma cell line and Jurkat human acute T cell leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for SMAC/Diablo at approximately 29 kDa (as indicated) using 5 µg/mL of Rabbit Anti-Human SMAC/Diablo Antigen Affinity-purified Polyclonal Antibody (Catalog # AF789). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SMAC/Diablo

SMAC (second mitochondria derived activator of caspase)/Diablo promotes caspase activation by interacting with the inhibitor of apoptosis (IAP) proteins in the cytochrome c/Apaf-1/caspase-9 pathway.

References
  1. Du, C. et al. (2000) Cell 102:33.
  2. Chai, J. et al. (2000) Nature 406:855.
  3. Srinivasula, S. et al. (2000) J. Biol. Chem. 275:36152.
  4. Ekert, P. et al. (2001) J. Cell Biol. 152:483.
  5. Verhagen, A. (2000) Cell 102:43.
Long Name
Second Mitochondria-derived Activator of Caspase
Entrez Gene IDs
56616 (Human)
Alternate Names
0610041G12Rik; Diablo; diablo, IAP-binding mitochondrial protein; Direct IAP-binding protein with low pI; mitochondrial Smac protein; Second mitochondria-derived activator of caspase; SMAC; SMACmitochondrial

Product Datasheets

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Citations for Human SMAC/Diablo Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. Theophylline and dexamethasone in combination reduce inflammation and prevent the decrease in HDAC2 expression seen in monocytes exposed to cigarette smoke extract
    Authors: Xue‑Jiao Sun, Zhan‑Hua Li, Yang Zhang, Xiao‑Ning Zhong, Zhi‑Yi He, Ji‑Hong Zhou et al.
    Experimental and Therapeutic Medicine
  2. Pro-Apoptotic Activity of New Honokiol/Triphenylmethane Analogues in B-Cell Lymphoid Malignancies
    Molecules, 2016-07-30;21(8):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Proteasome inhibition can impair caspase-8 activation upon submaximal stimulation of apoptotic tumor necrosis factor-related apoptosis inducing ligand (TRAIL) signaling.
    Authors: Laussmann MA, Passante E, Hellwig CT
    J. Biol. Chem., 2012-03-09;287(18):14402-11.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Role and regulation of nodal/activin receptor-like kinase 7 signaling pathway in the control of ovarian follicular atresia.
    Authors: Wang H, Jiang JY, Zhu C, Peng C, Tsang BK
    Mol. Endocrinol., 2006-05-18;20(10):2469-82.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. Inhibition of lymphotoxin-beta receptor-mediated cell death by survivin-DeltaEx3.
    Authors: You RI, Chou YC
    Cancer Res., 2006-03-15;66(6):3051-61.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  6. High levels of X-linked Inhibitor-of-Apoptosis Protein (XIAP) are indicative of radio chemotherapy resistance in rectal cancer
    Authors: L. Flanagan, J. Kehoe, J. Fay, O. Bacon, A.U. Lindner, E.W. Kay et al.
    Radiation Oncology
  7. Systems analysis of apoptosis protein expression allows the case-specific prediction of cell death responsiveness of melanoma cells
    Authors: E Passante, M L Würstle, C T Hellwig, M Leverkus, M Rehm
    Cell Death & Differentiation

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Reviews for Human SMAC/Diablo Antibody

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Human SMAC/Diablo Antibody
By Anonymous on 08/18/2017
Application: WB Sample Tested: RAW 264.7 mouse monocyte/macrophage cell line Species: Mouse
Western Blot Human SMAC/Diablo Antibody AF789