Human SLITRK5 Antibody

Catalog #: AF2587 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF2587
AF2587-SP

All SLITRK5 Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human SLITRK5 Antibody Summary

Species Reactivity

Human

Specificity

Detects human SLITRK5 in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross-reactivity with recombinant human (rh) SLITRK1, rhSLITRK2, rhSLITRK3, rhSLITRK4 and rhSLITRK6 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human SLITRK5
Glu39-Val665
Accession # O94991

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human SLITRK5 (Catalog # 2587-SK)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SLITRK5

SLITRK5 (Slit and Trk-like family member 5; also named LRR-containing protein 11) is a member of the SLITRK family of proteins (1, 2). This family currently includes six members, all of which contain a slit-like extracellular region and (save for SLITRK1) a Trk-like cytoplasmic region (3). Human SLITRK5 is a type I transmembrane (TM) protein that is 958 amino acids (aa) in length. It contains a putative 40 aa signal sequence, a 624 aa extracellular domain (ECD), a 21 aa TM segment, and a 273 aa cytoplasmic region. The ECD is characterized by the presence of two leucine-rich domains (LRD) that resemble those found in slit. Each LRD (aa 80‑285 plus aa 384‑579) contains seven and eight leucine-rich repeats (LRRs), respectively. The exact function of the LRRs is not known. Based on other LRRs, it might be assumed that they mediate protein-protein-interaction (4). The cytoplasmic region contains two conserved, potential phosphorylation sites at Tyr833 and Tyr917, plus a TrkA-like tyrosine phosphorylation site (YLxxL) at Tyr945. SLITRK5 is expressed on embryonic neurons of the hippocampus (pyramidal layer) and thalamus (3). Based on SLITRK2 studies, SLITRK5 will block neurite extension (3). Human SLITRK5 shares 97% and 98% aa identity with mouse and canine SLITRK5, respectively.

References

Aruga, J. et al. (2003) Gene 315:87.
Nagase, T. et al. (2001) DNA Res. 8:179.
Aruga, J. and K. Mikoshiba (2003) Mol. Cell. Neurosci. 24:117.
Enkhbayar, P. et al. (2003) Proteins 54:394.

Long Name

SLIT and NTRK-like Family, Member 5

Entrez Gene IDs

26050 (Human); 75409 (Mouse); 306152 (Rat)

Alternate Names

bA364G4.2; FLJ58374; KIAA0918; leucine-rich repeat-containing protein 11; LRRC11; SLIT and NTRK-like family, member 5; SLIT and NTRK-like protein 5; SLITRK5