Human SLC22A1 Antibody

Catalog #: MAB6469 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB6469
MAB6469-SP

Detection of Human SLC22A1 by Western Blot.

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All SLC22A1 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human SLC22A1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human SLC22A1 in Western blots.

Source

Monoclonal Mouse IgG_2A Clone # 609222

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with human SLC22A1
Met1-Thr554
Accession # NP_003048

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Flow Cytometry

0.25 µg/10⁶ cells

HEK293 Human Cell Line Transfected with Human SLC22A1 and eGFP

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human SLC22A1 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line. PVDF Membrane was probed with 2 µg/mL of Human SLC22A1 Monoclonal Antibody (Catalog # MAB6469) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for SLC22A1 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Flow Cytometry

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Detection SLC22A1 in HEK293 Human Cell Line Transfected with Human SLC22A1 and eGFP by Flow Cytometry HEK293 human embryonic kidney cell line transfected with either (A) human SLC22A1 or (B) irrelevant protein and eGFP was stained with Mouse Anti-Human SLC22A1 Monoclonal Antibody (Catalog # MAB6469) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). Quadrant markers were set based on Mouse IgG2A control antibody (IC003). Staining was performed using our Staining Membrane-associated Proteins protocol.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SLC22A1

SLC22A1 (solute carrier family 22 member 1), also called OCT1, is a 554 amino acid (aa), 70-80 kDa 12-transmembrane glycoprotein of the SLC22 family of polyspecific organic cation transporters (OCT). SLC22A1 is expressed in epithelial cells and some neurons, with detection on hepatocyte sinusoidal membranes and enterocyte basolateral membranes. It transports selected cations bidirectionally and is inhibited by non-transported cations. Human SLC22A1 shares 78% aa identity with mouse and rat SLC22A1. In humans, three inactive isoforms of 506, 483 and 353 aa, lack C-terminal exons 5, 5 and 6, or 3 and 6, respectively.

Long Name

Solute carrier family 22 member 1

Entrez Gene IDs

6580 (Human); 24904 (Rat)

Alternate Names

hOCT1; OCT1; oct1_cds; OCT1solute carrier family 22 member 1; Organic cation transporter 1; SLC22A1; solute carrier family 22 (organic cation transporter), member 1