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Human sFRP-2 Antibody

Catalog #: MAB6838
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB6838
MAB6838-SP
Detection of Human sFRP‑2 by Western Blot.
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Citations (2)
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Human sFRP-2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human sFRP-2 in direct ELISAs and Western blots. In direct ELISAs, 100% cross-reactivity with recombinant mouse (rm) sFRP-2 is observed and no cross-reactivity with recombinant human (rh) sFRP-1 or rhsFRP-5 is observed. In Western blots, no cross-reactivity with rhsFRP-1, -4, -5, or rmsFRP-2 is observed. 100% cross-reactivity with rhsFRP-3 is observed under non-reducing conditions only.
Source
Monoclonal Mouse IgG2B Clone # 755644
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human sFRP-2
Leu25-Cys295
Accession # Q96HF1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human sFRP-2 antibody by Western Blot. View Larger

Detection of Human sFRP‑2 by Western Blot. Western blot shows lysates of human adipose tissue and human lung tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human sFRP-2 Monoclonal Antibody (Catalog # MAB6838) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for sFRP-2 at approximately 33 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: sFRP-2

Secreted Frizzled Related Protein-2 (sFRP-2) belongs to a family of Wnt-binding proteins with homology to the ligand-binding domain of the Frizzled transmembrane Wnt receptors. The sFRP proteins are approximately 30‑35 kDa in size and contain an N-terminal Frizzled-like domain with 10 conserved cysteines and a Netrin-like C‑terminal domain (1, 2). Mature human sFRP-2, also known as SARP-1, SDF-5, and FRP-2, shares 99% aa sequence identity with mouse and rat sFRP-2 (3). sFRP‑2 is widely expressed during embryogenesis and in the adult in tissues including the eye, heart, lung, colon, intestine, smooth muscle, pancreas, prostate, testis, kidney, brain, teeth and joints, craniofacial mesenchyme, and preadipocytes (3‑6). Depending on the context, sFRP-2 can exert either positive or negative effects on Wnt signaling (7‑9). It also inhibits BMP-induced effects (8, 10). sFRP-2 can be incorporated into the extracellular matrix through interactions with Fibronectin and Integrin alpha 5 beta 1 (11). sFRP-2 plays a variety of roles during tissue morphogenesis including inhibition of the planar cell polarity pathway and myoblast and osteoblast differentiation (8, 10, 12, 13). sFRP-2 is also expressed in multiple myeloma and glioma in which it promotes tumorigenicity (10, 14). At physiological concentrations sFRP-2 enhances BMP-1 mediated proteolysis of Pro-Collagen I, whereas at higher concentrations it inhibits BMP-1 activity (15, 16). This difference is significant considering that sFRP-2 is up‑regulated in fibrotic areas of the heart following myocardial infarction (15, 16). Elevated levels of sFRP-2 then promote the recovery of cardiac function by reducing collagen deposition, remodeling, and calcification and by promoting the engraftment of mesenchymal stem cells into the heart (8, 15).

References
  1. Bovolenta, P. et al. (2008) J. Cell Sci. 121:737.
  2. van Amerongen, R. and R. Nusse (2009) Development 136:3205.
  3. Hu, E. et al. (1998) Biochem. Biophys. Res. Commun. 247:287.
  4. Rattner, A. et al. (1997) Proc. Natl. Acad. Sci. 94:2859.
  5. Melkonyan, H.S. et al. (1997) Proc. Natl. Acad. Sci. 94:13636.
  6. Leimeister, C. et al. (1998) Mech. Dev. 75:29.
  7. Galli, L.M. et al. (2006) Dev. Dyn. 235:681.
  8. Alfaro, M.P. et al. (2010) J. Biol. Chem. 285:35645.
  9. von Marschall, Z. and L.W. Fisher (2010) Biochem. Biophys. Res. Commun. 400:299.
  10. Oshima, T. et al. (2005) Blood 106:3160.
  11. Lee, J.-L. et al. (2004) J. Biol. Chem. 279:14602.
  12. Sugiyama, Y. et al. (2010) Dev. Biol. 338:193.
  13. Descamps, S. et al. (2008) Cell Tissue Res. 332:299.
  14. Roth, W. et al. (2000) Oncogene 19:4210.
  15. He, W. et al. (2010) Proc. Natl. Acad. Sci. 107:21110.
  16. Kobayashi, K. et al. (2009) Nat. Cell Biol. 11:46.
Long Name
Secreted Frizzled Related Protein 2
Entrez Gene IDs
6423 (Human); 20319 (Mouse)
Alternate Names
FRP2; FRP-2secreted apoptosis related protein 1; SARP-1; SARP1sFRP-2; SDF-5; Secreted apoptosis-related protein 1; secreted frizzled-related protein 2; sFRP2; sFRP-2

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Citations for Human sFRP-2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. sFRP2 in the aged microenvironment drives melanoma metastasis and therapy resistance
    Authors: A Kaur, MR Webster, K Marchbank, R Behera, A Ndoye, CH Kugel, VM Dang, J Appleton, MP O'Connell, P Cheng, AA Valiga, R Morissette, NB McDonnell, L Ferrucci, AV Kossenkov, K Meeth, HY Tang, X Yin, WH Wood, E Lehrmann, KG Becker, KT Flaherty, DT Frederick
    Nature, 2016-04-04;532(7598):250-4.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Development (Detection)
  2. sFRP2 Supersedes VEGF as an Age-related Driver of Angiogenesis in Melanoma, Affecting Response to Anti-VEGF Therapy in Older Patients.
    Authors: Fane, M E, Ecker, B L Et al.
    Clin Cancer Res

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