Home / Serpin F1/PEDF / Human Serpin F1/PEDF Biotinylated Antibody

Human Serpin F1/PEDF Biotinylated Antibody

Catalog #: BAF1177
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BAF1177
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Human Serpin F1/PEDF Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Serpin F1/PEDF in Western blots. In this format, approximately 40% cross-reactivity with recombinant mouse Serpin F1 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
recombinant human Serpin F1/PEDF
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Serpin F1/PEDF

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Serpin F1/PEDF

Serpin F1 (serine proteinase inhibitor-clade F #1; also PEDF, PIG35 and EPC-1) is a secreted, 50 kDa glycoprotein member of the clade F-subfamily, serpin superfamily of protease inhibitors.  It is expressed by diverse cell types such as retinal pigment and breast epithelium, fibroblasts, astrocytes and hepatocytes.  Serpin F1 circulates in blood and binds to type I collagen plus heparan sulfate.  Although it is a serpin, it belongs to a class of serpins that are nonprotease inhibiting, and instead is known to possess potent antiangiogenic and neurotrophic activity.  Human serpin F1 is 418 amino acids (aa) in length.  It contains a 19 aa signal sequence plus a 399 aa mature region that shows an NLS (aa 146-149), a neuroprotective motif (aa 354-359) and an antiangiogenesis segment (aa 387-411).  Phosphorylation occurs at multiple sites that affect bioactivity.  Over aa 44-418, human serpin F1 shares 87% aa identity with mouse serpin F1.        

Long Name
Serpine F1/Pigment Epithelium-derived Factor
Entrez Gene IDs
5176 (Human); 20317 (Mouse); 287526 (Rat)
Alternate Names
Cell proliferation-inducing gene 35 protein; EPC-1; EPC-1PIG35; PEDF; PEDFpigment epithelium-derived factor; pigment epithelium derived factor), member 1; proliferation-inducing protein 35; serine (or cysteine) proteinase inhibitor, clade F (alpha-2 antiplasmin; Serpin F1; serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epitheliumderived factor), member 1

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Citation for Human Serpin F1/PEDF Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Pigment epithelium-derived factor improves metabolic derangements and ameliorates dysregulation of adipocytokines in obese type 2 diabetic rats.
    Authors: Matsui T, Nishino Y, Ojima A, Maeda S, Tahara N, Yamagishi S
    Am J Pathol, 2014-02-13;184(4):1094-103.
    Species: Rat
    Sample Types: Serum
    Applications: Western Blot

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