Home / Semaphorin 3A / Human Semaphorin 3A Antibody

Human Semaphorin 3A Antibody

Catalog #: AF1250
4 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF1250
AF1250-SP
Product Details
Citations (4)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Semaphorin 3A Antibody Summary

Species Reactivity
Human
Specificity
Detects human Semaphorin 3A in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant human (rh) Semaphorin 3B, rhSemaphorin 3C, rhSemaphorin 3D, rhSemaphorin 3E and rhSemaphorin 3F is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Semaphorin 3A
Lys26-Val771 (Arg552Ala, Arg555Ala)
Accession # Q14563
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Semaphorin 3A Fc Chimera (Catalog # 1250-S3)
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
Human T cells treated with PHA

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Semaphorin 3A

The Semaphorins constitute a large family of secreted, GPI-anchored and transmembrane cell signaling molecules. Depending on their domain organization and species origin, these proteins can be classified into eight groups. To date, at least 19 vertebrate Semaphorins belonging to five groups (class 3 through 7) have been identified. All Semaphorins contain a conserved, 500 amino acid (aa) Sema domain at the amino terminus. Semaphorins are best known for their roles in axon guidance during neuronal development. Semaphorins are also expressed in non-neuronal tissues and are involved in angiogenesis, hematopoiesis, organogenesis, and the regulation of immune functions (1, 2).

Class 3 Semaphorins (Sema3) are secreted proteins containing a Sema domain, an immunoglobulin c2-like domain and a basic domain near the carboxyl tail. Sema3A (also referred to as SemaIII, SemD and Collapsin) cDNA predicts a 771 aa precursor protein with a putative 25 aa signal peptide (1‑3). Bioactive Sema3A is a disulfide-linked dimer (4). The bioactivity is increased after proteolytic processing by a furin-like endoprotease near the carboxy-terminus (1). The functional receptor complex for Sema3 is composed of two distinct transmembrane proteins: Neuropilin-1 (Npn-1) and Plexin-A. Npn-1 binds directly to Sema3A with high-affinity and confers specificity. Plexin-A interacts with Npn-1 to increase the affinity of the complex for Sema3A and serves as the signaling subunit in the receptor complex (1, 2, 5).

References
  1. Nakamura, F. et al. (2000) J Neurobiol. 44:219.
  2. Goshima, Y. et al. (2002) J. Clin. Invest. 109:993.
  3. Kolodkin, A.L. et al. (1993) Cell 75:1389.
  4. Koppel, A.M. et al. (1998) J. Biol. Chem. 273:15708.
  5. Yu, T.W. et al. (2001) Nature Neurosci. Supplement 4:1169.
  6. Luo Y. et al. (1993) Cell 75:217.
Entrez Gene IDs
10371 (Human); 20346 (Mouse); 29751 (Rat); 100620398 (Porcine); 102126862 (Cynomolgus Monkey)
Alternate Names
(semaphorin) 3A; coll-1; collapsin 1; Hsema-I; Hsema-III; MGC133243; sema domain, immunoglobulin domain (Ig), short basic domain, secreted; Sema III; SEMA1; Sema3A; SEMAD; SEMAIII; SEMAL; Semaphorin 3A; semaphorin D; Semaphorin III; semaphorin L; semaphorin-3A; SemD

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Citations for Human Semaphorin 3A Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Semaphorin 3A suppresses tumor growth and metastasis in mice melanoma model.
    Authors: Chakraborty G, Kumar S, Mishra R
    PLoS ONE, 2012-03-20;7(3):e33633.
  2. Truncated-semaphorin3A is a potential regulatory molecule to restore immune homeostasis in immune-mediated diseases
    Authors: N Eiza, O Kessler, A Sabag, G Neufeld, EY Jones, Z Vadasz
    Frontiers in Pharmacology, 2023-01-10;13(0):1085892.
    Species: Human
    Sample Types: Protein
    Applications: ELISA Detection, Western Blot
  3. Possible implication of satellite cells in regenerative motoneuritogenesis: HGF upregulates neural chemorepellent Sema3A during myogenic differentiation.
    Authors: Tatsumi R, Sankoda Y, Anderson JE, Sato Y, Mizunoya W, Shimizu N, Suzuki T, Yamada M, Rhoads RP, Ikeuchi Y, Allen RE
    Am. J. Physiol., Cell Physiol., 2009-06-10;297(2):C238-52.
    Species: Rat
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  4. Chromatin Association of XRCC5/6 in the Absence of DNA Damage Depends upon the XPE Gene Product DDB2.
    Authors: Fantini D, Huang S, Asara JM et al.
    Molecular Biology of The Cell.

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