Human Semaphorin 3A Antibody Summary
Lys26-Val771 (Arg555Ala, Arg552Ala)
Accession # Q14563
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Semaphorin 3A in U118-MG cells by Flow Cytometry U118-MG cells were stained with Mouse Anti-Human Semaphorin 3A Monoclonal Antibody (Catalog # MAB1250, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram) followed by Fluorescein-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0103B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Semaphorin 3A
The Semaphorins constitute a large family of secreted, GPI-anchored and transmembrane cell signaling molecules. Depending on their domain organization and species origin, these proteins can be classified into eight groups. To date, at least 19 vertebrate Semaphorins belonging to five groups (class 3 through 7) have been identified. All Semaphorins contain a conserved, 500 amino acid (aa) Sema domain at the amino terminus. Semaphorins are best known for their roles in axon guidance during neuronal development. Semaphorins are also expressed in non-neuronal tissues and are involved in angiogenesis, hematopoiesis, organogenesis, and the regulation of immune functions (1, 2).
Class 3 Semaphorins (Sema3) are secreted proteins containing a Sema domain, an immunoglobulin c2-like domain and a basic domain near the carboxyl tail. Sema3A (also referred to as SemaIII, SemD and Collapsin) cDNA predicts a 771 aa precursor protein with a putative 25 aa signal peptide (1‑3). Bioactive Sema3A is a disulfide-linked dimer (4). The bioactivity is increased after proteolytic processing by a furin-like endoprotease near the carboxy-terminus (1). The functional receptor complex for Sema3 is composed of two distinct transmembrane proteins: Neuropilin-1 (Npn-1) and Plexin-A. Npn-1 binds directly to Sema3A with high-affinity and confers specificity. Plexin-A interacts with Npn-1 to increase the affinity of the complex for Sema3A and serves as the signaling subunit in the receptor complex (1, 2, 5).
- Nakamura, F. et al. (2000) J Neurobiol. 44:219.
- Goshima, Y. et al. (2002) J. Clin. Invest. 109:993.
- Kolodkin, A.L. et al. (1993) Cell 75:1389.
- Koppel, A.M. et al. (1998) J. Biol. Chem. 273:15708.
- Yu, T.W. et al. (2001) Nature Neurosci. Supplement 4:1169.
- Luo Y. et al. (1993) Cell 75:217.
Product Datasheets
Citations for Human Semaphorin 3A Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 7
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A secreted proteomic footprint for stem cell pluripotency
Authors: Lewis, PA;Silajdi?, E;Smith, H;Bates, N;Smith, CA;Mancini, FE;Knight, D;Denning, C;Brison, DR;Kimber, SJ;
PloS one
Species: Human
Sample Types: Cell Culture Supernates
Applications: Western Blot -
A Potential Role of Semaphorin 3A during Orthodontic Tooth Movement
Authors: S ?en, CJ Lux, R Erber
International Journal of Molecular Sciences, 2021-08-02;22(15):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Regulation of antitumour CD8 T-cell immunity and checkpoint blockade immunotherapy by Neuropilin-1
Authors: M Leclerc, E Voilin, G Gros, S Corgnac, V de Montpré, P Validire, G Bismuth, F Mami-Choua
Nat Commun, 2019-07-26;10(1):3345.
Species: Human
Sample Types: Protein
Applications: Western Blot -
B7-H4 Modulates Regulatory CD4+ T Cell Induction and Function via Ligation of a Semaphorin 3a/Plexin A4/Neuropilin-1 Complex
Authors: JR Podojil, MY Chiang, I Ifergan, R Copeland, LN Liu, S Maloveste, S Langermann, D Liebenson, R Balabanov, H Chi, L Chen, DAA Vignali, SD Miller
J. Immunol., 2018-06-13;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Brain endothelial cells control fertility through ovarian-steroid-dependent release of semaphorin 3A.
Authors: Giacobini P, Parkash J, Campagne C, Messina A, Casoni F, Vanacker C, Langlet F, Hobo B, Cagnoni G, Gallet S, Hanchate N, Mazur D, Taniguchi M, Mazzone M, Verhaagen J, Ciofi P, Bouret S, Tamagnone L, Prevot V
PLoS Biol, 2014-03-11;12(3):e1001808.
Species: Mouse, Rat
Sample Types: Whole Tissue
Applications: Neutralization -
Semaphorin 3A suppresses tumor growth and metastasis in mice melanoma model.
Authors: Chakraborty G, Kumar S, Mishra R
PLoS ONE, 2012-03-20;7(3):e33633.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Measles virus modulates dendritic cell/T-cell communication at the level of plexinA1/neuropilin-1 recruitment and activity.
Authors: Tran-Van H, Avota E, Bortlein C, Mueller N, Schneider-Schaulies S
Eur. J. Immunol., 2011-01-01;41(1):151-63.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Western Blot
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