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Human S100B Antibody

Catalog #: MAB1820
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB1820
MAB1820-SP
Detection of Human S100B by Western Blot.
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Product Details
Citations (2)
FAQs
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human S100B Antibody Summary

Species Reactivity
Human
Specificity
Detects human S100B in direct ELISAs and Western blots. In Western blots, this antibody does not cross-react with recombinant human (rh) S100A1, 2, 6, 7, 10, 11, 13, 16, rmS100A4, 8, 9, or rhS100P.
Source
Monoclonal Mouse IgG2A Clone # 472806
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human S100B
Met1-Glu92
Accession # P04271
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Simple Western
10 µg/mL
Human brain (cerebellum) tissue

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human S100B antibody by Western Blot. View Larger

Detection of Human S100B by Western Blot. Western blot shows lysate of human brain (cerebellum) tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human S100B Monoclonal Antibody (Catalog # MAB1820) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for S100B at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western Detection of Human S100B antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human S100B by Simple WesternTM. Simple Western lane view shows lysates of human brain (cerebellum) tissue, loaded at 0.2 mg/mL. A specific band was detected for S100B at approximately 4 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human S100B Monoclonal Antibody (Catalog # MAB1820). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: S100B

S100B belongs to the S100 subgroup of the EF-hand family of Ca2+ binding proteins. It is a homodimer that is expressed primarily in the brain by astrocytes, oligodendrocytes and Schwann cells. S100B has multiple intracellular functions, but can also be secreted from cells to exert extracellular functions. Some of the extracellular functions of S100B may be mediated by RAGE (receptor for advanced glycation end products). Blood levels of S100B can be used to monitor the extent of brain injury and malignant melanoma.

Long Name
S100 Calcium Binding Protein B
Entrez Gene IDs
6285 (Human)
Alternate Names
beta (neural); NEF; S100 beta; S100 calcium binding protein B; S100 calcium-binding protein B; S100 calcium-binding protein, beta (neural); S-100 calcium-binding protein, beta chain, 10protein S100-B; S-100 protein beta chain; S-100 protein subunit beta; S100; S100B; S100beta

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Citations for Human S100B Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Endoplasmic reticulum protein 29 regulates epithelial cell integrity during the mesenchymal-epithelial transition in breast cancer cells.
    Authors: Bambang IF, Lee YK, Richardson DR et al.
    Oncogene
  2. Increased serum S100B levels in chronic schizophrenic patients on long-term clozapine or typical antipsychotics.
    Authors: Qi LY, Xiu MH, Chen da C, Wang F, Kosten TA, Kosten TR, Zhang XY
    Neurosci. Lett., 2009-06-17;462(2):113-7.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Development

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