Human Rictor Antibody

Catalog #: MAB4598 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB4598
MAB4598-SP

Detection of Human Rictor by Western Blot.

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Human Rictor Antibody Summary

Species Reactivity

Human

Specificity

Detects endogenous human Rictor in Western blots.

Source

Monoclonal Rat IgG_2B Clone # 454313

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human Rictor
Met1-Leu250
Accession # Q6R327

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Rictor by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Human Rictor Monoclonal Antibody (Catalog # MAB4598) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Rictor at approximately 200 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Rictor

Rictor (rapamycin-insensitive companion of mTOR) is a member of the mTORC2 complex, which regulates cell growth by integrating stress, energy, and nutrient signals with growth factor derived signals. Rictor contains no obvious catalytic motifs, but knockdown studies of mTOR or Rictor results in a loss actin polymerization and cell spreading. In vitro, the mTOR-Rictor complex phosphorylates Akt/PKB on Ser473, an essential step for full activation of Akt/PKB. Additionally, recent studies have shown that mTOR-Rictor regulates PKC alpha phosphorylation and PDGFR beta expression and signaling. Rictor has also been described as a protein substrate for Akt.

Long Name

Rapamycin-insensitive Companion of mTOR

Entrez Gene IDs

253260 (Human)

Alternate Names

AVO3 homolog; AVO3; hAVO3; KIAA1999DKFZp686B11164; mAVO3; MGC39830; PIA; Pianissimo; rapamycin-insensitive companion of mTOR; Rictor; RPTOR independent companion of MTOR, complex 2; TORC2-specific protein AVO3