Human Rev-erb beta /NR1D2 Antibody

Catalog #: PP-H2729-00
1 Citation Datasheet / COA / SDS

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PP-H2729-00

Product Details

Citations (1)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Rev-erb beta /NR1D2 Antibody Summary

Species Reactivity

Human

Specificity

This antibody specifically recognizes human Rev-Erb beta and cross-reacts with rat Rev-Erb beta. It does not recognize human Rev-Erb alpha. Not yet tested in other species.

Source

Monoclonal Mouse IgG₃ Clone # H2729

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Recombinant human Rev-erb beta/NR1D2
aa 4-101

Formulation

A liquid formulation in physiologic saline with 0.1% NaN₃.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

This antibody can be used at 1 μg/mL under reducingconditions with the appropriate secondary reagents to detect human Rev-Erb beta.

Immunohistochemistry

This antibody can be used at 50 - 100 μg/mL withthe appropriate secondary reagents to detect human Rev-Erb beta.

Immunoprecipitation

Optimal dilutions should be determined by eachlaboratory.

Direct ELISA

This antibody can be used at 0.3 μg/mL with the appropriatesecondary reagents to detect human Rev-Erb beta.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

This antibody is stable for greater than six months when held at -20 °C in a manual defrost freezer or at -70 °C. Upon thawing, the antibody can be stored at 2-8 °C for at least 1 month without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Background: Rev-erb beta/NR1D2

Reverse Orientation the c-ErbA-1 Gene beta (Rev-Erb beta, BD73; NR1D2) is a member of the Orphan Nuclear Receptor family. Rev-Erb beta is expressed in the heart, brain, lung, liver, skeletal muscles, kidney, spleen and testis. Rev-Erb beta plays a broad role in cell proliferation and physiology.

Entrez Gene IDs

9975 (Human)

Alternate Names

BD73; EAR-1R; HZF2; NR1D2; nuclear receptor subfamily 1 group D member 2; nuclear receptor subfamily 1, group D, member 2; Orphan nuclear hormone receptor BD73; Reverb beta; Rev-erb beta; rev-erba-alpha-related receptor; rev-erb-beta; RVR; V-erbA-related protein 1-related

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Steroid Hormone Receptors and Regulators

Citation for Human Rev-erb beta /NR1D2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

NR1D2 Accelerates Hepatocellular Carcinoma Progression by Driving the Epithelial-to-Mesenchymal Transition
Authors: H Tong, X Liu, T Li, W Qiu, C Peng, B Shen, Z Zhu
Onco Targets Ther, 2020-05-08;13(0):3931-3942.
Species: Human
Sample Types: Cell Lysates, Whole Tissue, Whole Cells
Applications: Flow Cytometry, IHC, Western Blot

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