Human Renin Propeptide Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Renin in Human Kidney. Renin was detected in immersion fixed paraffin-embedded sections of human kidney using 15 µg/mL Mouse Anti-Human Renin Propeptide Monoclonal Antibody (Catalog # MAB4447) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Background: Renin
Human Renin is a member of the aspartyl proteinase family produced largely in part by the juxtaglomerular cells in the kidney (1). Renin differs from the other members of this class by having a pH optimum near the neutral pH region with native substrates instead of a pH 2.0 to 3.4 range (2). This more neutral pH optimum allows it to be functional in the plasma. Renin also has a very high selectivity for substrates due to a long peptide recognition on either side of the peptide bond undergoing cleavage. An octapeptide substrate was the minimum length to be cleaved by Renin. Renin plays a crucial role in the regulation of blood pressure and salt balance through the cleavage of angiotensinogen, which is the only known physiological substrate of Renin. Renin releases the decapeptide angiotensin I, which in turn is further converted to vasoactive hormone angiotensin II by angiotensin converting enzyme (ACE). Renin is produced as prorenin with 43 pro residues at the N-terminal of mature Renin. The inactive prorenin becomes activated proteolytically by trypsin, cathepsin B, or other proteinases. Renin is expressed as a precursor consisting of a signal sequence (aa 1-23), a propeptide (aa 24-66), and a mature chain (aa 67-406). The amino acid sequence of human REN shares 100%, 73%, 71% and 67% identity with that of chimpanzee, canine, mouse and rat.
- Yokosawa, H. et al. (1980) J. Biol. Chem. 255:3498.
- Fuminaki, S. et al. (2004) in Handbook of Proteolytic Enzymes, Barret, A.J. et al. eds. p. 54.
Product Datasheets
Citations for Human Renin Propeptide Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Pathophysiological clinical features of an infant with hypertension secondary to multicystic dysplastic kidney: a case report
Authors: K Sugimoto, T Enya, K Joh, K Miyazaki, T Miyazawa, R Ohshima, S Marutani, T Tsukasa, M Okada
Bmc Nephrology, 2021-02-05;22(1):55.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Expression of renin angiotensin system (RAS) components in endometrial cancer
Authors: Sarah J Delforce
Endocr Connect, 2016-12-12;0(0):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P
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