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Human/Rat OV-6 APC-conjugated Antibody

Catalog #: FAB2020A
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB2020A
Detection of OV‑6 in Huh‑7 Human Cell Line by Flow Cytometry.
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Citations (3)
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Human/Rat OV-6 APC-conjugated Antibody Summary

Species Reactivity
Human, Rat
Specificity
Detects human and rat OV-6 antigen.
Source
Monoclonal Mouse IgG1 Clone # OV-6
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Carcinogen-treated rat nodular hepatocytes
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of OV‑6 antibody in Huh‑7 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of OV‑6 in Huh‑7 Human Cell Line by Flow Cytometry. Huh-7 human hepatoma cell line was stained with Mouse Anti-Human CD34 PE-conjugated Monoclonal Antibody (Catalog # FAB7227P) and either (A) Mouse Anti-Human/Rat OV-6 APC-conjugated Monoclonal Antibody (Catalog # FAB2020A) or (B) Mouse IgG1Allophycocyanin Isotype Control (Catalog # IC002A). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: OV-6

OV-6 antigen has been used as a marker to identify oval cells, putative hepatic stem cells that can differentiate into multiple cell types.

References
  1. Dunsford, H.A. and S. Sell (1989) Cancer Res. 49:4887.
  2. Parent, R. et al. (2004) Gastroenterology 126:1147.
  3. He, Z.P. et al. (2003) Cell Prolif. 37:177.
  4. Lazaro, C.A. et al. (2003) Hepatology 38:1095.
  5. He, Z.P. et al. (2003) Differentation 71:281.
Long Name
Oval Cell Marker
Alternate Names
OV6; OV-6

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Citations for Human/Rat OV-6 APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Co-Expression of CD34, CD90, OV-6 and Cell-Surface Vimentin Defines Cancer Stem Cells of Hepatoblastoma, Which Are Affected by Hsp90 Inhibitor 17-AAG
    Authors: M Lee-Theile, JR Hadhoud, G Volante, DD Fadini, J Eichhorn, U Rolle, HC Fiegel
    Cells, 2021-09-29;10(10):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. The transcription factor LEF1 promotes tumorigenicity and activates the TGF-? signaling pathway in esophageal squamous cell carcinoma
    Authors: Y Zhao, J Zhu, B Shi, X Wang, Q Lu, C Li, H Chen
    J. Exp. Clin. Cancer Res., 2019-07-11;38(1):304.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. PRMT1 regulates the tumour-initiating properties of esophageal squamous cell carcinoma through histone H4 arginine methylation coupled with transcriptional activation
    Authors: Y Zhao, Q Lu, C Li, X Wang, L Jiang, L Huang, C Wang, H Chen
    Cell Death Dis, 2019-05-01;10(5):359.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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