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Human/Rat OV-6 Antibody

Catalog #: MAB2020
26 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB2020
MAB2020-SP
OV‑6 in BG01V Human Embryonic Stem Cells.
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Citations (26)
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Human/Rat OV-6 Antibody Summary

Species Reactivity
Human, Rat
Specificity
Detects human and rat OV-6 antigen.
Source
Monoclonal Mouse IgG1 Clone # OV-6
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Carcinogen-treated rat nodular hepatocytes
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry

Parent, R. et al. (2004) Gastroenterology 126(4):1147.
He, Z.P. et al. (2003) Cell Prolif. 37(2):177.

 
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry OV-6 antibody in BG01V Human Embryonic Stem Cells by Immunocytochemistry (ICC). View Larger

OV‑6 in BG01V Human Embryonic Stem Cells. OV-6 was detected in immersion fixed BG01V human embryonic stem cells differentiated into hepatocytes using Mouse Anti-Human/Rat OV-6 Monoclonal Antibody (Catalog # MAB2020) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and cell surfaces. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: OV-6

OV-6 antigen has been used as a marker to identify oval cells, putative hepatic stem cells that can differentiate into multiple cell types.

References
  1. Dunsford, H.A. and S. Sell (1989) Cancer Res. 49:4887.
  2. Parent, R. et al. (2004) Gastroenterology 126:1147.
  3. He, Z.P. et al. (2003) Cell Prolif. 37:177.
  4. Lazaro, C.A. et al. (2003) Hepatology 38:1095.
  5. He, Z.P. et al. (2003) Differentation 71:281.
Long Name
Oval Cell Marker
Alternate Names
OV6; OV-6

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Citations for Human/Rat OV-6 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

26 Citations: Showing 1 - 10
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  1. Expression of the Pluripotency Transcription Factor OCT4 in the Normal and Aberrant Mammary Gland
    Authors: Foteini Hassiotou, Anna R. Hepworth, Adriana S. Beltran, Michelle M. Mathews, Alison M. Stuebe, Peter E. Hartmann et al.
    Frontiers in Oncology
  2. Inhibition of notch signaling pathway prevents cholestatic liver fibrosis by decreasing the differentiation of hepatic progenitor cells into cholangiocytes
    Authors: Xiao Zhang, Guangli Du, Ying Xu, Xuewei Li, Weiwei Fan, Jiamei Chen et al.
    Laboratory Investigation
  3. Hepatic insulin synthesis increases in rat models of diabetes mellitus type 1 and 2 differently
    Authors: Abidov, M;Sokolova, K;Danilova, I;Baykenova, M;Gette, I;Mychlynina, E;Aydin Ozgur, B;Gurol, AO;Yilmaz, MT;
    PloS one
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  4. Integration of OV6 expression and CD68+ tumor-associated macrophages with clinical features better predicts the prognosis of patients with hepatocellular carcinoma
    Authors: MD Wang, H Xiang, L Zhang, C Wang
    Translational Oncology, 2022-08-26;25(0):101509.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  5. Capsaicin suppresses hepatocarcinogenesis by inhibiting the stemness of hepatic progenitor cells via SIRT1/SOX2 signaling pathway
    Authors: ZQ Xie, HX Li, XJ Hou, MY Huang, ZM Zhu, LX Wei, CX Tang
    Cancer Medicine, 2022-06-08;0(0):.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  6. Hepatic stellate cells contribute to liver regeneration through galectins in hepatic stem cell niche
    Authors: JY Ge, YW Zheng, T Tsuchida, K Furuya, H Isoda, H Taniguchi, N Ohkohchi, T Oda
    Stem Cell Res Ther, 2020-09-29;11(1):425.
    Species: Rat
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC
  7. The transcription factor LEF1 promotes tumorigenicity and activates the TGF-? signaling pathway in esophageal squamous cell carcinoma
    Authors: Y Zhao, J Zhu, B Shi, X Wang, Q Lu, C Li, H Chen
    J. Exp. Clin. Cancer Res., 2019-07-11;38(1):304.
    Species: Human, Xenograft
    Sample Types: Whole Cells, Whole Tissue
    Applications: Flow Cytometry, IHC-P
  8. PRMT1 regulates the tumour-initiating properties of esophageal squamous cell carcinoma through histone H4 arginine methylation coupled with transcriptional activation
    Authors: Y Zhao, Q Lu, C Li, X Wang, L Jiang, L Huang, C Wang, H Chen
    Cell Death Dis, 2019-05-01;10(5):359.
    Species: Human, Xenograft
    Sample Types: Whole Cells, Whole Tissue
    Applications: Cell Sorting, IHC-P
  9. Cells responsible for liver mass regeneration in rats with 2-acetylaminofluorene/partial hepatectomy injury
    Authors: CS Chien, YH Chen, HL Chen, CP Wang, SH Wu, SL Ho, WC Huang, CH Yu, MH Chang
    J. Biomed. Sci., 2018-04-25;25(1):39.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  10. Embedded Disposable Functionalized Electrochemical Biosensor with a 3D-Printed Flow Cell for Detection of Hepatic Oval Cells (HOCs)
    Authors: S Damiati, M Peacock, S Leonhardt, L Damiati, MA Baghdadi, H Becker, R Kodzius, B Schuster
    Genes (Basel), 2018-02-14;9(2):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Selection
  11. Prognostic roles of pathology markers immunoexpression and clinical parameters in Hepatoblastoma
    Authors: JF Wu, HH Chang, MY Lu, ST Jou, KC Chang, YH Ni, MH Chang
    J. Biomed. Sci., 2017-08-29;24(1):62.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  12. Hepatitis B virus X protein promotes the stem-like properties of OV6(+) cancer cells in hepatocellular carcinoma
    Authors: C Wang, MD Wang, P Cheng, H Huang, W Dong, WW Zhang, PP Li, C Lin, ZY Pan, MC Wu, WP Zhou
    Cell Death Dis, 2017-01-19;8(1):e2560.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  13. Metformin prevents hepatocellular carcinoma development by suppressing hepatic progenitor cell activation in a rat model of cirrhosis
    Authors: DK DePeralta, L Wei, S Ghoshal, B Schmidt, GY Lauwers, M Lanuti, RT Chung, KK Tanabe, BC Fuchs
    Cancer, 2016-02-23;122(8):1216-27.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  14. Contribution of Mature Hepatocytes to Biliary Regeneration in Rats with Acute and Chronic Biliary Injury.
    Authors: Chen Y, Chen H, Chien C, Wu S, Ho Y, Yu C, Chang M
    PLoS ONE, 2015-08-26;10(8):e0134327.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  15. Hepatic Progenitor Cells Contribute to the Progression of 2-Acetylaminofluorene/Carbon Tetrachloride-Induced Cirrhosis via the Non-Canonical Wnt Pathway.
    Authors: Chen J, Zhang X, Xu Y, Li X, Ren S, Zhou Y, Duan Y, Zern M, Zhang H, Chen G, Liu C, Mu Y, Liu P
    PLoS ONE, 2015-06-18;10(6):e0130310.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  16. GSK-3beta suppresses the proliferation of rat hepatic oval cells through modulating Wnt/beta-catenin signaling pathway.
    Authors: Ji X, Xie Y, Zhong J, Xu Q, Zeng Q, Wang Y, Zhang Q, Shan Y
    Acta Pharmacol Sin, 2015-02-09;36(3):334-42.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC-P
  17. The CD133+CD44+ precancerous subpopulation of oval cells is a therapeutic target for hepatocellular carcinoma.
    Authors: Zheng, Yun-Wen, Tsuchida, Tomonori, Shimao, Taiki, Li, Bin, Takebe, Takanori, Zhang, Ran-Ran, Sakurai, Yu, Ueno, Yasuharu, Sekine, Keisuke, Ishibashi, Naoto, Imajima, Makiko, Tanaka, Takuji, Taniguchi, Hideki
    Stem Cells Dev, 2014-06-26;23(18):2237-49.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  18. Stable insulin-secreting ducts formed by reprogramming of cells in the liver using a three-gene cocktail and a PPAR agonist.
    Authors: Banga, A, Greder, L V, Dutton, J R, Slack, J M W
    Gene Ther, 2013-10-03;21(1):19-27.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  19. Effects of matrine on oval cellmediated liver regeneration and expression of RBPJkappa and HES1.
    Authors: Yang Z, Wang L, Hou Y, Wang X
    Mol Med Rep, 2013-03-27;7(5):1533-8.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC-P
  20. Expression and localization of regenerating gene I in a rat liver regeneration model.
    Authors: Wang J, Koyota S, Zhou X, Ueno Y, Ma L, Kawagoe M, Koizumi Y, Okamoto H, Sugiyama T
    Biochem. Biophys. Res. Commun., 2009-01-27;380(3):472-7.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  21. Two pathologic types of hepatocellular carcinoma with lymph node metastasis with distinct prognosis on the basis of CK19 expression in tumor.
    Authors: Zhuang PY, Zhang JB, Zhu XD, Zhang W, Wu WZ, Tan YS, Hou J, Tang ZY, Qin LX, Sun HC
    Cancer, 2008-06-15;112(12):2740-8.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  22. Wnt/beta-catenin signaling contributes to activation of normal and tumorigenic liver progenitor cells.
    Authors: Yang W, Yan HX, Chen L, Liu Q, He YQ, Yu LX, Zhang SH, Huang DD, Tang L, Kong XN, Chen C, Liu SQ, Wu MC, Wang HY
    Cancer Res., 2008-06-01;68(11):4287-95.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  23. Thy-1 is expressed in hepatic myofibroblasts and not oval cells in stem cell-mediated liver regeneration.
    Authors: Dezso K, Jelnes P, László V, Baghy K, Bödör C, Paku S, Tygstrup N, Bisgaard HC, Nagy P
    Am. J. Pathol., 2007-09-20;171(5):1529-37.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  24. Spatial Multiomics Analysis Reveals Only Minor Genetic and Epigenetic Changes in Human Liver Cancer Stem-Like Cells Compared With Other Tumor Parenchymal Cells
    Authors: Dan Liu, Hong Li, Hui Dong, Mincheng Qu, Liguang Yang, Lina Chen et al.
    Frontiers in Cell and Developmental Biology
  25. Wharton’s jelly-derived mesenchymal stem cells combined with praziquantel as a potential therapy for Schistosoma mansoni-induced liver fibrosis
    Authors: Olfat A. Hammam, Nagwa Elkhafif, Yasmeen M. Attia, Mohamed T. Mansour, Mohamed M. Elmazar, Rania M. Abdelsalam et al.
    Scientific Reports
  26. Effect of human umbilical cord blood stem cell transplantation on oval cell response in 2-AAF/CCL4 liver injury model: experimental immunohistochemical study
    Authors: Hussein Abdellatif, Gamal Shiha, Dalia M. Saleh, Huda Eltahry, Kamal G. Botros
    Inflammation and Regeneration

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