Human/Rat Histone Deacetylase 2/HDAC2 Antibody

Catalog # Availability Size / Price Qty
MAB7679
MAB7679-SP
Detection of Human and Rat Histone Deacetylase 2/HDAC2 by Western Blot.
2 Images
Product Details
Citations (2)
FAQs
Supplemental Products
Reviews (1)

Human/Rat Histone Deacetylase 2/HDAC2 Antibody Summary

Species Reactivity
Human, Rat
Specificity
Detects human Histone Deacetylase 2/HDAC-2 in direct ELISAs and human and rat Histone Deacetylase 2/HDAC-2 Western blots. In direct ELISAs, no cross-reactivity with recombinant human HDAC1 is observed.
Source
Monoclonal Mouse IgG2B Clone # 806116
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Histone Deacetylase 2/HDAC-2
Pro386-Pro488
Accession # Q92769
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.2 µg/mL
See below
Simple Western
2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human and Rat Histone Deacetylase 2/HDAC2 antibody by Western Blot. View Larger

Detection of Human and Rat Histone Deacetylase 2/HDAC2 by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, SH-SY5Y human neuroblastoma cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human Histone Deacetylase 2/HDAC2 Monoclonal Antibody (Catalog # MAB7679) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Histone Deacetylase 2/HDAC2 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western Detection of Human Histone Deacetylase 2/HDAC2 antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human Histone Deacetylase 2/HDAC2 by Simple WesternTM. Simple Western lane view shows lysates of K562 human chronic myelogenous leukemia cell line, loaded at 0.5 mg/mL. A specific band was detected for Histone Deacetylase 2/HDAC2 at approximately 98 kDa (as indicated) using 2 µg/mL of Mouse Anti-Human/Rat Histone Deacetylase 2/HDAC2 Monoclonal Antibody (Catalog # MAB7679). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Loading...
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Histone Deacetylase 2/HDAC2

Histone deacetylase 2 (HDAC2), is a ubiquitously expressed intracellular enzyme that is involved in the post-translational modification of proteins. Acetyl groups are added to e-NH3 on lysine residues by acetyltransferases and removed by HDACs. The balance between these reactions has widespread effects on the enzymatic activity, subcellular localization, and molecular interactions of the substrates. HDAC2 can associate into several multiprotein complexes with discrete functions. Acetylation of core histones (H2A, H2B, H3, and H4) alters their interaction with DNA and the local availability of DNA for transcription. The function of HDAC2 itself is regulated by several post-translational modifications including phosphorylation, acetylation, ubiquitination, tyrosine nitrosylation, and cysteine carbonylation. Within aa 386-488, human HDAC2 shares 98% and 96% aa sequence identity with mouse and rat HDAC2, respectively.

Long Name
Histone Deacetylase 2
Entrez Gene IDs
3066 (Human); 15182 (Mouse); 84577 (Rat)
Alternate Names
EC 3.5.1.98; HD2; HDAC2; Histone Deacetylase 2; RPD3; transcriptional regulator homolog RPD3; YAF1; YY1-associated factor 1

Product Datasheets

You must select a language.

x

Citations for Human/Rat Histone Deacetylase 2/HDAC2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
Filter your results:

Filter by:

  1. Class I-Histone Deacetylase (HDAC) Inhibition is Superior to pan-HDAC Inhibition in Modulating Cisplatin Potency in High Grade Serous Ovarian Cancer Cell Lines
    Authors: Bandolik JJ, Hamacher A, Schrenk C et al.
    International Journal of Molecular Sciences
  2. Theophylline and dexamethasone in combination reduce inflammation and prevent the decrease in HDAC2 expression seen in monocytes exposed to cigarette smoke extract
    Authors: Xue‑Jiao Sun, Zhan‑Hua Li, Yang Zhang, Xiao‑Ning Zhong, Zhi‑Yi He, Ji‑Hong Zhou et al.
    Experimental and Therapeutic Medicine

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Human/Rat Histone Deacetylase 2/HDAC2 Antibody

Average Rating: 4 (Based on 1 Review)

5 Star
0%
4 Star
100%
3 Star
0%
2 Star
0%
1 Star
0%

Have you used Human/Rat Histone Deacetylase 2/HDAC2 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Human/Rat Histone Deacetylase 2/HDAC2 Antibody
By Anonymous on 01/23/2023
Application: Simple Western Sample Tested: Cell Lysates Species: Human

This experiment was conducted under reducing conditions, using the 12-230 kDa separation system, Jurkat whole cell lysates (loaded at 0.6 mg/ml protein concentration), HDAC2 antibody 1:100 (Catalog # MAB7679). Multiple bands detected: 65, 100 and 141 kDa.