Human/Rat CaMKK alpha Antibody

Catalog # Availability Size / Price Qty
AF4536
AF4536-SP
Detection of Human/Rat CaMKK alpha  by Western Blot.
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Human/Rat CaMKK alpha Antibody Summary

Species Reactivity
Human, Rat
Specificity
Detects endogenous human and rat CaMKK alpha in Western blots. In Western blots, this antibody does not cross-react with recombinant human CaMKK beta.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human CaMKK alpha
Met1-Ser132
Accession # Q8N5S9
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
10 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human/Rat CaMKKa antibody by Western Blot. View Larger

Detection of Human/Rat CaMKK alpha by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, CCD-1070Sk human foreskin fibroblast cell line, K562 human chronic myelogenous leukemia cell line, and human brain tissue. PVDF membrane was probed with 1 µg/mL of Human/Rat CaMKKa Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4536) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CaMKK alpha at approximately 68 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.

Simple Western Detection of Human CaMKKa antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human CaMKK alpha by Simple WesternTM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for CaMKKa at approximately 68 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human/Rat CaMKKa Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4536) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CaMKK alpha

The calcium/calmodulin (CaM) kinase signaling cascade includes the upstream kinases CaM kinase kinase alpha (CaMKK alpha ; also known as CaMKK1) and CaMKK beta (CaMKK2), and their substrates, the output kinases CaMKI and CaMKIV. Largely by activation of the transcription factor CREB, the CaM kinase cascade is implicated in neuronal development and long-term memory formation. In addition, CaMKK alpha is known to modulate skeletal muscle glucose uptake, and is upregulated during retinoic acid-induced neutrophil maturation.

Long Name
Calcium/Calmodulin-dependent Protein Kinase Kinase alpha
Entrez Gene IDs
84254 (Human); 55984 (Mouse); 60341 (Rat)
Alternate Names
calcium/calmodulin-dependent protein kinase kinase 1; calcium/calmodulin-dependent protein kinase kinase 1, alpha; Calcium/calmodulin-dependent protein kinase kinase alpha; CaM-kinase IV kinase; CaM-kinase kinase 1; CaM-kinase kinase alpha; CaM-KK 1; CAMKK alpha protein; CaMKK alpha; CaM-KK alpha; CaMKK1; CAMKKACaMKK 1; DKFZp761M0423; EC 2.7.11; EC 2.7.11.17; MGC34095

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