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Human RAR alpha /NR1B1 Antibody

Catalog #: PP-H1920-00
1 Citation Datasheet / COA / SDS
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PP-H1920-00
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Citations (1)
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Human RAR alpha /NR1B1 Antibody Summary

Species Reactivity
Human
Specificity
This antibody specifically recognizes human RAR alpha and does not cross-react with human RAR beta or RAR gamma. Not yet tested in other species.
Source
Monoclonal Mouse IgG1 Clone # H1920
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Recombinant human RAR alpha/NR1B1
Formulation
A liquid formulation in physiologic saline with 0.1% NaN3.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
This antibody can be used at 1 μg/mL under reducingconditions and at 3 μg/mL under non-reducing conditions with the appropriatesecondary reagents to detect human RAR alpha.
 
Immunoprecipitation
Optimal dilutions should be determined by eachlaboratory.
 
Direct ELISA
This antibody can be used at 0.2 μg/mL with the appropriatesecondary reagents to detect human RAR alpha.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
This antibody is stable for greater than six months when held at -20 °C in a manual defrost freezer or at -70 °C. Upon thawing, the antibody can be stored at 2-8 °C for at least 1 month without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Background: RAR alpha/NR1B1

Retinoic Acid Receptor alpha (RAR alpha ; NR1B1) is closely related to the Thyroid hormone Receptor (TR). RARs bind to two retinoids, all-trans retinoic acid and 9-cis retinoic acid. RAR alpha is expressed in adult skin, and lung. RAR alpha is redundantly involved in vertebrates in the pleiotropic control of embryonic patterning and organogenesis, cell proliferation, differentiation and apoptosis, as well as homeostatic control.

Long Name
Retinoic Acid Receptor, alpha
Entrez Gene IDs
5914 (Human)
Alternate Names
alpha polypeptide; NR1B1; RAR alpha; RARA; RAR-alpha; retinoic acid receptor, alpha

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Citation for Human RAR alpha /NR1B1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Identification of a binding motif specific to HNF4 by comparative analysis of multiple nuclear receptors
    Authors: Bin Fang, Daniel Mane-Padros, Eugene Bolotin, Tao Jiang, Frances M. Sladek
    Nucleic Acids Research

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