Human PSMA/FOLH1/NAALADase I Antibody

Catalog #: MAB42342 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB42342-100
MAB42342-SP

Detection of PSMA/FOLH1 in LNCaP Human Cell Line by Flow Cytometry.

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Human PSMA/FOLH1/NAALADase I Antibody Summary

Species Reactivity

Human

Specificity

Detects Human PSMA/FOLH1/NAALADase I in direct ELISAs

Source

Monoclonal Mouse IgG_2A Clone # 460407

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human PSMA/FOLH1/NAALADase I
Lys44-Ala750
Accession # Q04609

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

LNCaP human prostate cancer cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of PSMA/FOLH1 in LNCaP Human Cell Line by Flow Cytometry. LNCaP human prostate cancer cell line was stained with Mouse Anti-Human PSMA/FOLH1 Monoclonal Antibody (Catalog # MAB42342, filled histogram) or Mouse IgG2A isotype control antibody (MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (F0102B). Staining was performed using our Staining Membrane-associated Proteins protocol.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PSMA/FOLH1/NAALADase I

Human prostate-specific membrane antigen (PSMA), a tumor marker in prostate cancer encoded by the FOLH1 gene, is a type II transmembrane zinc metallopeptidase that is most highly expressed in the nervous system, prostate, kidney, and small intestine (1, 2). The enzyme is also known as glutamate carboxypeptidase II (GCPII), folate hydrolase 1, folypoly-gamma-glutamate carboxypeptidase (FGCP), and N-acetylated-alpha-linked acidic dipeptidase I (NAALADase I). In the brain, PSMA hydrolyzes the neurotransmitter N-acetyl-Asp-Glu to produce glutamate, another neurotransmitter. Inhibition of brain PSMA activity is considered to be a promising approach for the treatment of neurological disorders associated with glutamate excitotoxicity, such as stroke, chronic pain, and amyotrophic lateral sclerosis (3). Intestinal PSMA hydrolyzes folylpoly-gamma -glutamates, facilitating the uptake of folate (4).

References

Silver, D.A. et al. (1997) Clin. Cancer Res. 3:81.
Carter, R.E. et al. (1996) Pro. Natl. Acad. Sci. USA. 93:749.
Jackson, P.F. and Slusher, B.S. (2001) Curr. Med. Chem. 8:949.
Heston, W.D. (1997) Urology 49:104.

Long Name

Prostate-specific Membrane Antigen

Entrez Gene IDs

2346 (Human); 53320 (Mouse); 85309 (Rat); 101866140 (Cynomolgus Monkey)

Alternate Names

Cell growth-inhibiting gene 27 protein; cell growth-inhibiting protein 27; EC 3.4.17.21; FGCP; folate hydrolase (prostate-specific membrane antigen) 1; Folate hydrolase 1; FOLH1; FOLHNAALADase I; Folylpoly-gamma-glutamate carboxypeptidase; GCP2; GCPII; GCPIImGCP; glutamate carboxylase II; glutamate carboxypeptidase 2; Glutamate carboxypeptidase II; Membrane glutamate carboxypeptidase; mopsm; NAALAD1; NAALAD1N-acetylated-alpha-linked acidic dipeptidase I; NAALADase I; NAALAdase; N-acetylated alpha-linked acidic dipeptidase 1; prostate specific membrane antigen variant F; Prostate-specific membrane antigen; PSMA; PSMAFGCP; PSMPteroylpoly-gamma-glutamate carboxypeptidase