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Human Prolactin Antibody

Catalog #: MAB6821
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB6821
MAB6821-SP
Detection of Human Prolactin by Western Blot.
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Citations (1)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Prolactin Antibody Summary

Species Reactivity
Human
Specificity
Recognizes both the full-length (amino acids 29 - 229) and a truncated form (amino acids 29 - 151) of recombinant human Prolactin in direct ELISAs and Western blots.
Source
Monoclonal Mouse IgG1 Clone # 127813
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Prolactin
Leu29-Thr151
Accession # P01236
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Prolactin antibody by Western Blot. View Larger

Detection of Human Prolactin by Western Blot. Western blot shows lysate of human pituitary tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Prolactin Monoclonal Antibody (Catalog # MAB6821) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Prolactin at approximately 23 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Prolactin

Prolactin (PRL) is a neuroendocrine pituitary hormone that is synthesized by anterior pituitary, placenta, brain, uterus, dermal fibroblasts, decidua, B cells, T cells, natural killer cells, and breast cancer cells. The prolactin receptor is a transmembrane type I glycoprotein that belongs to the hematopoietic cytokine receptor family.

Prolactin receptor (PRL R) contains an extracellular, transmembrane, and intracellular domain. Transcriptional regulation of the PRL R gene results in several different species-dependent isoforms of PRL R being produced.

Entrez Gene IDs
5617 (Human); 19109 (Mouse)
Alternate Names
PRL; Prolactin

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Citation for Human Prolactin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Prolactin receptor antagonism reduces the clonogenic capacity of breast cancer cells and potentiates doxorubicin and paclitaxel cytotoxicity.
    Authors: Howell SJ, Anderson E, Hunter T
    Breast Cancer Res., 2008-08-05;10(4):R68.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Western Blot

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