Human Progesterone R B/NR3C3 Antibody

Catalog # Availability Size / Price Qty
MAB5886
MAB5886-SP
Detection of Human Progesterone R B/NR3C3 by Western Blot.
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Human Progesterone R B/NR3C3 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Progesterone R B in Western blots.
Source
Monoclonal Mouse IgG2B Clone # 569012
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Progesterone R B
Met1-Leu189
Accession # P06401
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunohistochemistry
5-25 µg/mL
See below
Immunocytochemistry
3-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Progesterone R B/NR3C3 antibody by Western Blot. View Larger

Detection of Human Progesterone R B/NR3C3 by Western Blot. Western blot shows lysates of T47D human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Progesterone R B/NR3C3 Monoclonal Antibody (Catalog # MAB5886) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Progesterone R B/NR3C3 at approximately 118 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Immunocytochemistry Progesterone R B/NR3C3 antibody in T47D Human Cell Line by Immunocytochemistry (ICC). View Larger

Progesterone R B/NR3C3 in T47D Human Cell Line. Progesterone R B/NR3C3 was detected in immersion fixed T47D human breast cancer cell line using Mouse Anti-Human Progesterone R B/NR3C3 Monoclonal Antibody (Catalog # MAB5886) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry Progesterone R B/NR3C3 antibody in Human Prostate Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

Progesterone R B/NR3C3 in Human Prostate Cancer Tissue. Progesterone R B/NR3C3 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Mouse Anti-Human Progesterone R B/NR3C3 Monoclonal Antibody (Catalog # MAB5886) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Progesterone R B/NR3C3

Progesterone Receptor B (PR-B) is a 118 kDa member of the NR3 subfamily within the nuclear hormone receptor family of proteins. It is expressed in several tissues, including female reproductive organs as well as neurons throughout the CNS. PR-B is particularly important in the mammary gland where it mediates proliferative responses to progesterone. Human PR-B contains an N-terminal regulatory region (aa1‑566), a DNA binding domain (aa 567‑639), and a steroid-binding region (aa 681‑933). Ligand binding induces a key phosphorylation event at Ser294 by ERK1/2.

Long Name
Progesterone Receptor B
Entrez Gene IDs
5241 (Human)
Alternate Names
PGRB; Progesterone R B; Progesterone RB

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Human Progesterone R B/NR3C3 Antibody
By Anonymous on 12/01/2021
Application: IHC Sample Tested: Ovarian carcinoma Species: Human