Human/Primate SLC6A3/DAT1 Extracellular Loop 2 Antibody

Catalog #: PPS069 Datasheet / COA / SDS

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PPS069

Detection of SLC6A3/DAT1 (Extracellular Loop 2) by Western Blot.

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Human/Primate SLC6A3/DAT1 Extracellular Loop 2 Antibody Summary

Species Reactivity

Human, Primate

Specificity

Specific for the ~88 kDa DAT protein in Western blots of SDS-solubilized human striatal samples and in IHC applications with
formaldehyde-fixed human and monkey brain sections.

Source

Polyclonal Rabbit IgG

Purification

Antigen Affinity-purified

Immunogen

SLC6A3/DAT1

Formulation

100 μL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50% glycerol.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1:1000 dilution

See below

Immunohistochemistry

1:1000 dilution

(frozen sections)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of SLC6A3/DAT1 (Extracellular Loop 2) by Western Blot. Western blot of SDS-solubilized human caudate (20 μg protein) showing the ~88 kDa band representing the DAT protein.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

For long-term storage, ≤ -20° C is recommended. Product is stable at ≤ -20° C for at least 1 year.

Background: SLC6A3/DAT1

The dopamine transporter (DAT) is a 70-80 kDa member of the Na⁺-neurotransmitter symporter family of transmembrane (TM) proteins. DAT plays a crucial role in the synaptic clearance of dopamine (DA). It mediates the uptake of dopamine by the presynaptic terminal, thus limiting the strength of the dopamingeric response. One molecule of DA is accompanied by two Na⁺ and one Cl^- ion. Molecules such as amphetamine both competitively inhibit DA uptake, and induce DA release through the DAT, increasing the rewarding property of DA. Human DAT is a 620 amino acid (aa), 12 TM phosphoglycoprotein with an N- and C-terminal cytoplasmic domain. It exists as a disulfide-linked oligomer on the cell surface. Phosphorylation of the N-terminus (S7/12) promotes DA release. The C-terminus binds CaMKII, as well as Hic5, Pick1, and synuclein which regulate receptor trafficking and expression. There is an extended 71 aa extracellular (EC) loop between TM segments 3 and 4. Glycosylation at this site is necessary for oligomer expression on the cell membrane. The human 42 aa DAT C-terminus is 93% aa identical to the mouse DAT
C-terminus. The human EC loop is also 93% aa identical to the rhesus monkey EC loop.

References

Torres, G.E. (2006) J. Neurochem. 97(Suppl. 1):3.
Sotnikova, T.D. et al. (2006) CNS Neurol. Disord. Drug Targets. 5:45.
Fog, J.U. et al. (2006) Neuron 51:417.
Giros, B. et al. (1992) Mol. Pharmacol. 42:383.
Cervinski, M.A. et al. (2005) J. Biol. Chem. 280:40442.

Long Name

Dopamine Transporter

Entrez Gene IDs

6531 (Human); 13162 (Mouse); 24898 (Rat)

Alternate Names

DA transporter; DAT1; DATDAT1Solute carrier family 6 member 3; SLC6A3; sodium-dependent dopamine transporter; solute carrier family 6 (neurotransmitter transporter, dopamine), member 3; VNTR