Human Phospho-Rad17 (S635) Antibody

Name: Human Phospho-Rad17 (S635) Antibody
Brand: R&D Systems
SKU: AF1374
Price: 367 USD

Catalog #: AF1374 Datasheet / COA / SDS

Discontinued Product

AF1374 has been discontinued.

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Detection of Human Phospho-Rad17 (S635) by Western Blot.

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Human Phospho-Rad17 (S635) Antibody Summary

Species Reactivity

Human

Specificity

Detects human Rad17 when phosphorylated at S635.

Source

Polyclonal Rabbit IgG

Purification

Antigen Affinity-purified

Immunogen

Phosphopeptide containing human Rad17 S635 site

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS and Sodium Azide with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Phospho-Rad17 (S635) by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line mock treated or exposed to 10 Gy IR or 100 J/m²UV-C for 1 hour. PVDF membrane was probed with 0.5 µg/mL Rabbit Anti-Human Phospho-Rad17 (S635) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1374) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for Phospho-Rad17 (S635) was detected at approximately 92 kDa (as indicated). The phospho-specificity of this antibody was supported by decreased labeling following treatment with 600 U lambda-phosphatase (lambda-PPase) for 30 minutes time. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Rad17

The DNA damage response activates cell cycle checkpoints to allow time for DNA repair and ensure the fidelity of each cell cycle. One group of proteins that transduce the DNA damage signal is the Rad family. The hRad17 protein is phosphorylated at S635 in cells exposed to multiple types of genotoxic stress, including ionizing radiation (IR) and ultraviolet light (UV).

Entrez Gene IDs

5884 (Human); 310034 (Rat)

Alternate Names

CCYC; cell cycle checkpoint protein (RAD17); cell cycle checkpoint protein RAD17; FLJ41520; HRAD17; R24L; RAD1 (S. pombe) homolog; RAD1 homolog; RAD17 homolog (S. pombe); Rad17; Rad17-like protein; RAD17SP; RAD24; RF-C activator 1 homolog; RF-C/activator 1 homolog