Human Phospho-p53 (S392) Antibody

Detection of Human Phospho-p53 (S392) by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line untreated (-) or treated (+) with 1 µM camptothecin (CPT) for 5 hours. PVDF membrane was probed with 0.1 µg/mL Rabbit Anti-Human Phospho-p53 (S392) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2996) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for Phospho-p53 (S392) was detected at approximately 53 kDa (as indicated). The phospho-specificity of this antibody was supported by decreased labeling following treatment with 600 U lambda-phosphatase (lambda-PPase) for 1 hour. For additional reference, the membrane was stripped and reprobed with 1:5000 dilution of Human/Mouse/Rat p53 HRP-conjugated Antigen Affinity-purified Polyclonal Antibody (lower panel, Catalog # HAF1355). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human Phospho-p53 (S392) by Simple Western^TM. Simple Western lane view shows lysates of MCF‑7 human breast cancer cell line untreated (-) or treated (+) with 1 µM Camptothecin (CPT) for 5 hours, loaded at 0.5 mg/mL. A specific band was detected for Phospho-p53 (S392) at approximately 62 kDa (as indicated) using 1 µg/mL of Rabbit Anti-Human Phospho-p53 (S392) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2996). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.