Human Perforin Antibody

Catalog #: MAB103852 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB103852-100
MAB103852-SP

Detection of Human Perforin by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Perforin Antibody Summary

Species Reactivity

Human

Specificity

Detects human Perforin in direct ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 1031721

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese Hamster Ovary cell line CHO-derived human Perforin
Pro22-Trp555
Accession # P14222

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

NK‑92 human natural killer lymphoma cell line and NK‑RL human natural killer lymphocytic leukemia cell line

Immunohistochemistry

5-25 µg/mL

Immersion fixed paraffinembedded sections of human spleen

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Perforin by Western Blot. Western blot shows lysates of NK‑92 human natural killer lymphoma cell line and NK‑RL human natural killer lymphocytic leukemia cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Perforin Monoclonal Antibody (Catalog # MAB103852) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for Perforin at approximately 61 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Immunohistochemistry

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Perforin in Human Spleen. Perforin was detected in immersion fixed paraffin-embedded sections of human spleen using Mouse Anti-Human Perforin Monoclonal Antibody (Catalog # MAB103852) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Perforin

Perforin is a pore forming, cytolytic protein that is a component of cytolytic T-cell and NK cell granules. Perforin activates clathrin- and dynamin-dependent endocytosis, which removes perforin and granzymes from the plasm membrane to early endosomes, preserving outer membrane integrity. Perforin facilitates granzyme uptake and avoids the proinflammatory necrotic death of a membrane-damaged cell. Defects in the Perforin gene results in Familial hemophagocytic lymphohistiocytosis (FHL). Perforin-based effector systems are involved not only in the lysis of abnormal cells but also in the down-regulation of cellular immune activation.

Long Name

Perforin 1 (Pore Forming Protein)

Entrez Gene IDs

5551 (Human); 18646 (Mouse); 50669 (Rat)

Alternate Names

Cytolysin; FLH2; HPLH2; HPLH2lymphocyte pore forming protein; Lymphocyte pore-forming protein; MGC65093; P1; P1PFN1; perforin 1 (pore forming protein); Perforin; perforin-1; PFP; PFPcytolysin; PRF1