Human Peptidase Inhibitor 16/PI16 Antibody

Catalog #: AF4980 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF4980
AF4980-SP

All Peptidase Inhibitor 16/PI16 Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Peptidase Inhibitor 16/PI16 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Peptidase Inhibitor 16/PI16 in direct ELISAs and Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Peptidase Inhibitor 16/PI16
Leu28-Leu437
Accession # AAH35634

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human Peptidase Inhibitor 16/PI16

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Peptidase Inhibitor 16/PI16

PI16 (Peptidase Inhibitor 16; also PSP94-binding protein) is a 70‑75 kDa member of the CRISP family of proteins. It is expressed by prostatic fibroblasts and cardiomyocytes and is known to bind PSP94. The human PI16 precursor is 463 amino acids (aa) in length. It contains a 27 aa signal peptide plus a 436 aa mature region. The mature region has been described as being either a type I transmembrane (TM) glycoprotein with a TM segment between aa 443‑462, or a GPI‑linked glycoprotein with C‑terminal cleavage between Lys437‑Asn438. Three potential variants exist. One shows a deletion of aa 231‑423, a second shows a 9 aa substitution for the C‑terminal 64 aa and a third shows a 43 aa insertion after Cys197. Over aa 28‑437, human PI16 is 67% aa identical to mouse PI16.

Entrez Gene IDs

221476 (Human); 74116 (Mouse); 294312 (Rat)

Alternate Names

CRISP9; CRISP-9; Cysteine-rich secretory protein 9; dJ90K10.5; DKFZp586B1817; MGC45378; microseminoprotein, beta-binding protein; MSMBBP; Peptidase Inhibitor 16; PI16; PI-16; protease inhibitor 16; PSP94-binding protein; PSPBP