Home / PDGF R beta / Human PDGF R beta Antibody

Human PDGF R beta Antibody

Catalog #: MAB1263
21 Citations 4 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB1263
MAB1263-SP
Detection of PDGF R beta in MG-63 cells by Flow Cytometry
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Product Details
Citations (21)
FAQs
Supplemental Products
Reviews (4)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human PDGF R beta Antibody Summary

Species Reactivity
Human
Specificity
Detects human PDGF R beta in Western blots. Specificity has been confirmed in binding studies using several different cell lines (1, 2) and by its ability to immunoprecipitate PDGF receptor beta ‑subunit complexed with 125I‑PDGF-BB (3). It does not recognize the PDGF receptor alpha ‑subunit. Detects the PDGF receptor beta ‑subunit of human and primate species (monkey and baboon) but not the rat or mouse receptors. Its ability to bind to receptors from other species has not been tested.
Source
Monoclonal Mouse IgG1 Clone # PR7212
Purification
Protein A or G purified from ascites
Immunogen
Human skin fibroblast membrane extracts
Formulation
Supplied as a 0.2 μm filtered solution in PBS. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human PDGF R beta Fc Chimera (Catalog # 385-PR)
Flow Cytometry
0.25 µg/106 cells
MG-63 human osteosarcoma cell line
Immunoprecipitation
Gronwald et al. (1988) Proc. Natl. Acad. Sci. 85:3435.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry View Larger

Detection of PDGF R beta in MG-63 cells by Flow Cytometry MG-63 cells were stained with Mouse Anti-Human PDGF R beta Monoclonal Antibody (Catalog # MAB1263, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C, as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 6 months, -20 to -70 °C under sterile conditions after opening.

Background: PDGF R beta

PDGF is a major serum mitogen that can exist as a homo or hetero-dimeric protein consisting of disulfide-linked PDGF-A and PDGF‑B chains. The PDGF-AA, PDGF‑BB and PDGF-AB isoforms have been shown to bind to two distinct cell surface PDGF receptors with different affinities. Where as PDGF R alpha binds all three PDGF isoforms with high affinity, PDGF R beta binds PDGF-BB only with high-affinity. Both PDGF R alpha and PDGF R beta  are members of the class III subfamily of receptor tyrosine kinases (RTK) that also includes the receptors for M-CSF, SCF and Flt3 ligand. All class III RTKs are characterized by the presence of five immuno‑globulinlike domains in their extracellular region and a split kinase domain in their intracellular region. PDGF binding induces receptor homo-and hetero‑dimerization and signal transduction. The expression of the alpha and beta receptors is independently regulated in various cell types. Recombinant soluble PDGF R beta binds PDGF with high affinity and is potent PDGF antagonist (4).

References
  1. Hart et al. (1987) J. Biol. Chem. 262:10780.
  2. Gronwald et al. (1988) Proc. Natl. Acad. Sci. 85:3435.
  3. Seifert et al. (1989) J. Biol. Chem. 264:8771.
  4. Heldin, C.H. and L. Claesson-Welsh (1994) in Guidebook to Cytokines and Their Receptors, Nicola, N.A. ed. Oxford University Press, New York, p. 202.
Long Name
Platelet-derived Growth Factor Receptor beta
Entrez Gene IDs
5159 (Human); 18596 (Mouse); 24629 (Rat)
Alternate Names
beta-type platelet-derived growth factor receptor; CD140 antigen-like family member B; CD140b antigen; CD140b; EC 2.7.10; EC 2.7.10.1; JTK12; PDGF R beta; PDGFR; PDGFR1; PDGFRB; PDGF-R-beta; platelet-derived growth factor receptor, beta polypeptide

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Citations for Human PDGF R beta Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

21 Citations: Showing 1 - 10
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  1. Generation of vascularized human cardiac organoids for 3D in vitro modeling
    Authors: Holly K. Voges, Richard J. Mills, Enzo R. Porrello, James E. Hudson
    STAR Protocols
  2. Proteomics profiling identifies extracellular vesicles' cargo associated with tumour cell induced platelet aggregation
    Authors: N McNamee, LR de la Fuen, MJ Santos-Mar, L O'Driscoll
    BMC Cancer, 2022-09-29;22(1):1023.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Regeneration of collagen fibrils at the papillary dermis by reconstructing basement membrane at the dermal-epidermal junction
    Authors: S Iriyama, Y Ogura, S Nishikawa, J Hosoi, S Amano
    Scientific Reports, 2022-01-17;12(1):795.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  4. Single-cell RNA sequencing reveals the mesangial identity and species diversity of glomerular cell transcriptomes
    Authors: B He, P Chen, S Zambrano, D Dabaghie, Y Hu, K Möller-Hac, D Unnersjö-J, GG Korkut, E Charrin, M Jeansson, M Bintanel-M, A Witasp, L Wennberg, A Wernerson, B Schermer, T Benzing, P Ernfors, C Betsholtz, M Lal, R Sandberg, J Patrakka
    Nature Communications, 2021-04-09;12(1):2141.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  5. Comparing the Effect of TGF-&beta Receptor Inhibition on Human Perivascular Mesenchymal Stromal Cells Derived from Endometrium, Bone Marrow and Adipose Tissues
    Authors: S Gurung, D Ulrich, M Sturm, A Rosamilia, JA Werkmeiste, CE Gargett
    Journal of personalized medicine, 2020-12-01;10(4):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  6. Synergistic activity of agents targeting growth factor receptors, CDKs and downstream signaling molecules in a panel of pancreatic cancer cell lines and the identification of antagonistic combinations: Implications for future clinical trials in pancreatic cancer
    Authors: T Khan, AM Seddon, AG Dalgleish, S Khelwatty, N Ioannou, S Mudan, H Modjtahedi
    Oncol Rep, 2020-10-22;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Fibroblasts direct differentiation of human breast epithelial progenitors
    Authors: M Morsing, J Kim, R Villadsen, N Goldhammer, A Jafari, M Kassem, OW Petersen, L Rønnov-Jes
    Breast Cancer Res., 2020-09-29;22(1):102.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  8. A novel sensitive assay for detection of a biomarker of pericyte injury in cerebrospinal fluid
    Authors: Melanie D. Sweeney, Abhay P. Sagare, Maricarmen Pachicano, Michael G. Harrington, Elizabeth Joe, Helena C. Chui et al.
    Alzheimer's & Dementia
  9. Atypical chemokine receptor ACKR3/CXCR7 controls postnatal vasculogenesis and arterial specification by mesenchymal stem cells via Notch signaling
    Authors: ST Wei, YC Huang, ML Hsieh, YJ Lin, WC Shyu, HC Chen, CH Hsieh
    Cell Death Dis, 2020-05-04;11(5):307.
    Species: Human
    Sample Types: Cell Culture Supernates
  10. GPRC5b Modulates Inflammatory Response in Glomerular Diseases via NF-kappa B Pathway
    Authors: Sonia Zambrano, Katja Möller-Hackbarth, Xidan Li, Patricia Q. Rodriguez, Emmanuelle Charrin, Angelina Schwarz et al.
    Journal of the American Society of Nephrology
  11. Spatial and temporal characterization of endometrial mesenchymal stem-like cells activity during the menstrual cycle
    Authors: William S B Yeung
    Exp. Cell Res., 2016-11-24;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  12. A phosphorylation switch controls the spatiotemporal activation of Rho GTPases in directional cell migration
    Authors: Xuan Cao, Tomonori Kaneko, Jenny S. Li, An-Dong Liu, Courtney Voss, Shawn S. C. Li
    Nature Communications
  13. The tyrosine phosphatase SHP-2 controls urokinase-dependent signaling and functions in human vascular smooth muscle cells.
    Authors: Kiyan J, Haller H, Dumler I
    Exp. Cell Res., 2008-12-25;315(6):1029-39.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  14. PDGF-C and -D induced proliferation/migration of human RPE is abolished by inflammatory cytokines.
    Authors: Li R, Maminishkis A, Wang FE, Miller SS
    Invest. Ophthalmol. Vis. Sci., 2007-12-01;48(12):5722-32.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  15. Ubiquitinylation of Ig beta dictates the endocytic fate of the B cell antigen receptor.
    Authors: Zhang M, Veselits M, O'Neill S, Hou P, Reddi AL, Berlin I, Ikeda M, Nash PD, Longnecker R, Band H, Clark MR
    J. Immunol., 2007-10-01;179(7):4435-43.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry, ICC
  16. Mesenchymal stem cell abnormalities in patients with multiple myeloma.
    Authors: Garderet L, Mazurier C, Chapel A, Ernou I, Boutin L, Holy X, Gorin NC, Lopez M, Doucet C, Lataillade JJ
    Leuk. Lymphoma, 2007-10-01;48(10):2032-41.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  17. Vascular endothelial growth factor can signal through platelet-derived growth factor receptors.
    Authors: Ball SG, Shuttleworth CA, Kielty CM
    J. Cell Biol., 2007-04-30;177(3):489-500.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  18. The in vitro migration capacity of human bone marrow mesenchymal stem cells: comparison of chemokine and growth factor chemotactic activities.
    Authors: Ponte AL, Marais E, Gallay N, Langonne A, Delorme B, Herault O, Charbord P, Domenech J
    Stem Cells, 2007-03-29;25(7):1737-45.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  19. Efficient induction of oligodendrocytes from human embryonic stem cells.
    Authors: Kang SM, Cho MS, Seo H, Yoon CJ, Oh SK, Choi YM, Kim DW
    Stem Cells, 2006-10-19;25(2):419-24.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  20. In vitro cytotoxic effects of imatinib in combination with anticancer drugs in human prostate cancer cell lines.
    Authors: Kubler HR, van Randenborgh H, Treiber U, Wutzler S, Battistel C, Lehmer A, Wagenpfeil S, Hartung R, Paul R
    Prostate, 2005-06-01;63(4):385-94.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  21. Expression of PDGF and their receptors in human retinal pigment epithelial cells and fibroblasts: regulation by TGF-beta.
    Authors: Nagineni CN, Kutty V, Detrick B, Hooks JJ
    J. Cell. Physiol., 2005-04-01;203(1):35-43.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Reviews for Human PDGF R beta Antibody

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