Human PDGF R alpha Antibody Summary
Supplied as a 0.2 μm filtered solution in PBS.
*Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of PDGF R alpha in MG-63 Human Cell Line by Flow Cytometry. MG-63 human osteosarcoma cell line was stained with Mouse Anti-Human PDGF Ra Monoclonal Antibody (Catalog # MAB1264, filled histogram) or isotype control antibody (MAB002, open histogram) followed by anti-Mouse IgG PE-conjugated Secondary Antibody (F0102B). Staining was performed using our Staining Membrane-associated Proteins protocol.
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Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C, as supplied.
- 1 month, 2 to 8 °C under sterile conditions after opening.
- 6 months, -20 to -70 °C under sterile conditions after opening.
Background: PDGF R alpha
PDGF is a major serum mitogen that can exist as a homo- or heterodimeric protein consisting of disulfide-linked PDGF-A and PDGF-B chains. The PDGF-AA, PDGF‑BB and PDGF-AB isoforms have been shown to bind to two distinct cell surface PDGF receptors with different affinities. Whereas PDGF R alpha binds all three PDGF isoforms with high affinity, PDGF R beta binds PDGF‑BB and AB, but not PDGF-AA. Both PDGF R alpha and PDGF R beta are members of the class III subfamily of receptor tyrosine kinases (RTK) that also includes the receptors for M-CSF, SCF and Flt3 ligand. All class III RTKs are characterized by the presence of five immunoglobulin-like domains in their extracellular region and a split kinase domain in their intracellular region. PDGF binding induces receptor homo-and heterodimerization and signal transduction. The expression of the alpha and beta receptors is independently regulated in various cell types. Only PDGF R alpha is expressed in oligodendrocyte progenitor cells, mesothelial cell and liver endothelial cells. Soluble PDGF-R alpha has been detected in cell conditioned medium and human plasma. Recombinant soluble PDGF R alpha binds PDGF with high affinity and is a potent PDGF antagonist (1).
- Heldin, C.H. and L. Claesson-Welsh (1994) Guidebook to Cytokines and Their Receptors, Nicola, N.A. (ed) Oxford University Press, New York, NY p. 202.
Product Datasheets
Citations for Human PDGF R alpha Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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PDGFRA K385 mutants in myxoid glioneuronal tumors promote receptor dimerization and oncogenic signaling
Authors: de Villenfagne, L;Sablon, A;Demoulin, JB;
Scientific reports
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Synergistic activity of agents targeting growth factor receptors, CDKs and downstream signaling molecules in a panel of pancreatic cancer cell lines and the identification of antagonistic combinations: Implications for future clinical trials in pancreatic cancer
Authors: T Khan, AM Seddon, AG Dalgleish, S Khelwatty, N Ioannou, S Mudan, H Modjtahedi
Oncol Rep, 2020-10-22;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
A reference collection of patient-derived cell line and xenograft models of proneural, classical and mesenchymal glioblastoma
Authors: BW Stringer, BW Day, RCJ D'Souza, PR Jamieson, KS Ensbey, ZC Bruce, YC Lim, K Goasdoué, C Offenhäuse, S Akgül, S Allan, T Robertson, P Lucas, G Tollesson, S Campbell, C Winter, H Do, A Dobrovic, PL Inglis, RL Jeffree, TG Johns, AW Boyd
Sci Rep, 2019-03-20;9(1):4902.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Alveolar macrophages are critical for the inhibition of allergic asthma by mesenchymal stromal cells.
Authors: Mathias, Louisa J, Khong, Sacha M, Spyroglou, Lisa, Payne, Natalie, Siatskas, Christop, Thorburn, Alison N, Boyd, Richard, Heng, Tracy S
J Immunol, 2013-11-18;191(12):5914-24.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
PDGFRA alterations in cancer: characterization of a gain-of-function V536E transmembrane mutant as well as loss-of-function and passenger mutations.
Authors: Velghe A, Van Cauwenberghe S, Polyansky A, Chand D, Montano-Almendras C, Charni S, Hallberg B, Essaghir A, Demoulin J
Oncogene, 2013-06-10;33(20):2568-76.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Human glial-restricted progenitors survive, proliferate, and preserve electrophysiological function in rats with focal inflammatory spinal cord demyelination
Authors: Piotr Walczak, Angelo H. All, Nidhi Rumpal, Michael Gorelik, Heechul Kim, Anil Maybhate et al.
Glia
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Mesenchymal stem cell abnormalities in patients with multiple myeloma.
Authors: Garderet L, Mazurier C, Chapel A, Ernou I, Boutin L, Holy X, Gorin NC, Lopez M, Doucet C, Lataillade JJ
Leuk. Lymphoma, 2007-10-01;48(10):2032-41.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Ubiquitinylation of Ig beta dictates the endocytic fate of the B cell antigen receptor.
Authors: Zhang M, Veselits M, O'Neill S, Hou P, Reddi AL, Berlin I, Ikeda M, Nash PD, Longnecker R, Band H, Clark MR
J. Immunol., 2007-10-01;179(7):4435-43.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
Vascular endothelial growth factor can signal through platelet-derived growth factor receptors.
Authors: Ball SG, Shuttleworth CA, Kielty CM
J. Cell Biol., 2007-04-30;177(3):489-500.
Species: Human
Sample Types: Cell Lysates
Applications: Immunoprecipitation -
The in vitro migration capacity of human bone marrow mesenchymal stem cells: comparison of chemokine and growth factor chemotactic activities.
Authors: Ponte AL, Marais E, Gallay N, Langonne A, Delorme B, Herault O, Charbord P, Domenech J
Stem Cells, 2007-03-29;25(7):1737-45.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
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