Human PDGF-D Biotinylated Antibody

Catalog #: BAF1159 Datasheet / COA / SDS

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BAF1159

All PDGF-D Antibodies

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Human PDGF-D Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human PDGF-D in Western blots. In this format, less than 1% cross-reactivity with recombinant human (rh) PDGF-AA, rhPDGF-AB and rhPDGF-BB is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human PDGF-D

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human PDGF-D

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PDGF-D

The platelet-derived growth factor (PDGF) family consists of four disulfide-linked homodimers and one heterodimer (PDGF-AB). These proteins regulate diverse cellular functions through interactions with PDGF R alpha and R beta (1, 2). Mature PDGF-DD associates with PDGF R beta and triggers signaling through PDGF R beta homodimers and PDGF R alpha / beta heterodimers (3 - 5). The human PDGF-DD cDNA encodes a 370 amino acid (aa) precursor that includes a 23 aa signal sequence, one CUB domain, and one PDGF/VEGF domain (3, 4). The PDGF/VEGF domain shares 27 - 35% aa sequence identity with the corresponding regions of other PDGF family members. Human PDGF-DD shares 87% aa sequence identity with mouse and rat PDGF-DD. PDGF-DD is secreted as a100 kDa latent homodimer which is activated by proteolysis to release a 35 kDa bioactive protein containing the PDGF/VEGF homology domain (3,4,6,7). A splice variant of PDGF-DD has a 6 aa deletion near the N-terminus. A 72 aa deletion within the PDGF/VEGF domain generates an inactive protein in mouse but has not been detected in human (8). PDGF-DD is widely expressed in embryonic and adult tissues (3, 9, 10), and PDGF R beta is expressed in a generally complementary pattern (9, 11, 12). PDGF-DD functions as a growth factor for renal artery smooth muscle cells and lens epithelial cells, and as a macrophage chemoattractant (5, 9 - 11). PDGF-DD is overexpressed in and contributes to several disease states, including renal and hepatic fibrosis, mesangial proliferative glomerulopathy, pulmonary lymphoid infiltration, and many cancers (6, 11 - 15). PDGF-DD functions in both paracrine and autocrine manners (6, 7, 14).

References

Reigstad, L.J. et al. (2005) FEBS J. 272:5723.
Fredriksson, L. et al. (2004) Cytokine Growth Factor Rev. 15:197.
LaRochelle, W.J. et al. (2001) Nat. Cell Biol. 3:517.
Bergsten, E. et al. (2001) Nat. Cell Biol. 3:512.
Uutela, M. et al. (2004) Blood 104:3198.
Ustach, C.V. and H-R.C. Kim (2005) Mol. Cell. Biol. 25:6279.
Ustach, C.V. et al. (2004) Canc. Res. 64:1722.
Zhuo, Y. et al. (2003) Biochem. Biophys. Res. Commun. 308:126.
Changsirikulchai, S. et al. (2002) Kid. Int. 62:2043.
Ray, S. et al. (2005) J. Biol. Chem. 280:8494.
Hudkins, K.L. et al. (2004) J. Am. Soc. Nephrol. 15:286.
Lokker, N.A. et al. (2002) Canc. Res. 62:3729.
Taneda, S. et al. (2003) J. Am. Soc. Nephrol. 14:2544.
LaRochelle, W.J. et al. (2002) Canc. Res. 62:2468.
Xu, L. et al. (2005) Canc. Res. 65:5711.

Long Name

Platelet-derived Growth Factor D

Entrez Gene IDs

80310 (Human); 71785 (Mouse)

Alternate Names

IEGFMSTP036; Iris-expressed growth factor; PDGFD; PDGF-D; platelet derived growth factor D; SCDGF-BMGC26867; SCDGFBplatelet-derived growth factor D; spinal cord derived growth factor B; Spinal cord-derived growth factor B; spinal cord-derived growth factor-B