Human PD-ECGF/Thymidine Phosphorylase Antibody

Catalog #: AF1143 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF1143
AF1143-SP

Detection of Human PD-ECGF/Thymidine Phosphorylase by Western Blot.

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All PD-ECGF/Thymidine Phosphorylase Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human PD-ECGF/Thymidine Phosphorylase Antibody Summary

Species Reactivity

Human

Specificity

Detects human PD‑ECGF/Thymidine Phosphorylase in direct ELISAs and Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human PD‑ECGF/Thymidine Phosphorylase
Ala11-Gln482
Accession # P19971

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

See below

Immunohistochemistry

5-15 µg/mL

See below

Neutralization

Measured by its ability to neutralize PD‑ECGF/Thymidine Phosphorylase-induced proliferation in HUVEC human umbilical vein endothelial cells. Usuki, K. et al. (1990) Cell Regulation 1:577. The Neutralization Dose (ND₅₀) is typically 0.5-2.0 µg/mL in the presence of 150 ng/mL Recombinant Human PD‑ECGF/Thymidine Phosphorylase.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human PD-ECGF/Thymidine Phosphorylase by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human PD-ECGF/Thymidine Phosphorylase Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1143) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for PD-ECGF/Thymidine Phosphorylase at approximately 49 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

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PD‑ECGF/Thymidine Phosphorylase in Human Prostate Cancer Tissue. PD-ECGF/Thymidine Phosphorylase was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using 5 µg/mL Goat Anti-Human PD-ECGF/Thymidine Phosphorylase Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1143) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counter-stained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Neutralization

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Cell Proliferation Induced by PD‑ECGF/Thymidine Phosphorylase and Neutralization by Human PD‑ECGF/Thymidine Phosphorylase Antibody. Recombinant Human PD-ECGF/Thymidine Phosphorylase (Catalog # 229-PE) stimulates proliferation in HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human PD-ECGF/Thymidine Phosphorylase (150 ng/mL) is neutralized (green line) by increasing concentrations of Human PD-ECGF/Thymidine Phosphorylase Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1143). The ND₅₀ is typically 0.5-2.0 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PD-ECGF/Thymidine Phosphorylase

PD-ECGF, also known as TPase and gliostatin, is produced by placenta, platelets, liver, lung, spleen, lymph nodes, and peripheral lymphocytes. It is overexpressed by many tumors in response to chemical or physical stress. PD-ECGF promotes endothelial cell proliferation and chemotaxis and promotes angiogenesis. PD-ECGF is a key enzyme in the pyrimidine nucleoside salvage pathway. It also metabolizes and inactivates chemotherapeutic drugs such as 5-fluorouracil and its derivatives. The human PD-ECGF cDNA encodes a 482 amino acid (aa) polypeptide with a 10 aa propeptide. The protein is localized mostly within the producer cells. N-terminal truncation, resulting in proteins lacking 10 aa and 6 aa, has been observed in PD-ECGF purified from platelets and placenta, respectively. In solution, PD-ECGF exists as a non-disulfide linked homodimer. The latter protein was purified from quiescent astrocytes and described as an astrocyte and astrocytoma cell growth inhibitor based on its ability to inhibit ³H-thymidine incorporation.

References

Haraguchi, M. et al. (1994) Nature 368:198.
Toi, M. et al. (2005) Lancet Oncol. 6:158.
Akiyama, S. et al. (2004) Canc. Sci. 95:851.

Long Name

Platelet-derived Endothelial Cell Growth Factor

Entrez Gene IDs

1890 (Human); 72962 (Mouse)

Alternate Names

dThdPase; EC 2.4.2; EC 2.4.2.4; ECGF; ECGF1; ECGF1TP; endothelial cell growth factor 1 (platelet-derived); Gliostatin; hPD-ECGF; MEDPS1; MNGIE; MTDPS1; PDECGF; PD-ECGF; Platelet-derived endothelial cell growth factor; TdRPase; Thymidine Phosphorylase; TPase; TYMP