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Human PD-1 Antibody

Catalog #: AF1086
34 Citations 6 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF1086
AF1086-SP
Detection of Human PD‑1 by Western Blot.
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Product Details
Citations (34)
FAQs
Supplemental Products
Reviews (6)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human PD-1 Antibody Summary

Specificity
Detects human PD-1 in ELISAs and Western blots. In sandwich ELISAs, less than 2% cross-reactivity with recombinant mouse PD‑1 and less than 0.2% cross-reactivity with recombinant human (rh) CD28, rhICOS, and rhCTLA‑4 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human PD-1
Leu25-Gln167
Accession # Q8IX89
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5-2 µg/mL
See below
Flow Cytometry
0.25 µg/106 cells
See below
Immunohistochemistry
5-15 µg/mL
See below
Blockade of Receptor-ligand Interaction
In a functional ELISA, 3-12 µg/mL of this antibody will block 50% of the binding of 500 ng/mL of Recombinant Human B7-H1 Fc Chimera (Catalog # 156-B7) to immobilized Recombinant Human PD-1 Fc Chimera (Catalog # 1086-PD) coated at 1 µg/mL (100 µL/well). At 30 μg/mL, this antibody will block >90% of the binding.
 
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Human PD-1 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
0.2-0.8 µg/mL 

Use in combination with:

Detection Reagent: Human PD‑1 Biotinylated Antibody (Catalog # BAF1086)

Standard: Recombinant Human PD-1 Fc Chimera Protein, CF (Catalog # 1086-PD)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human PD‑1 antibody by Western Blot. View Larger

Detection of Human PD‑1 by Western Blot. Western blot shows lysate of human thymus tissue. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human PD-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1086) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for PD-1 at approximately 40-50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western Blot Detection of Human PD-1 antibody by Western Blot. View Larger

Detection of Human PD‑1 by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line either mock transfected or transfected with human PD-1. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human PD-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1086) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for PD-1 at approximately 40-80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Flow Cytometry Detection of PD-1 antibody in Human PBMCs treated with PHA antibody by Flow Cytometry. View Larger

Detection of PD‑1 in Human PBMCs treated with PHA by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) either (A) untreated or (B) treated with 5 µg/mL PHA overnight were stained with Goat Anti-Human PD-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1086) followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107) and Mouse Anti-Human CD3e APC-conjugated Monoclonal Antibody (Catalog # FAB100A). Quadrant markers were set based on control antibody staining (Catalog # F0107). View our protocol for Staining Membrane-associated Proteins.

Immunohistochemistry PD-1 antibody in Human Lymph Node by Immunohistochemistry (IHC-P). View Larger

PD‑1 in Human Lymph Node. PD-1 was detected in immersion fixed paraffin-embedded sections of human lymph node using Goat Anti-Human PD-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1086) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PD-1

Programmed Death-1 (PD-1) is a type I transmembrane protein belonging to the CD28/CTLA-4 family of immunoreceptors that mediate signals for regulating immune responses (1). Members of the CD28/CTLA-4 family have been shown to either promote T cell activation (CD28 and ICOS) or down-regulate T cell activation (CTLA-4 and PD-1) (2). PD-1 is expressed on activated T cells, B cells, myeloid cells, and on a subset of thymocytes. In vitro, ligation of PD-1 inhibits TCR-mediated T-cell proliferation and production of IL-1, IL-4, IL-10, and IFN-gamma. In addition, PD-1 ligation also inhibits BCR mediated signaling. PD-1 deficient mice have a defect in peripheral tolerance and spontaneously develop autoimmune diseases (2, 3).

Two B7 family proteins, PD-L1 (also called B7-H1) and PD-L2 (also known as B7-DC), have been identified as PD-1 ligands. Unlike other B7 family proteins, both
PD‑L1 and PD-L2 are expressed in a wide variety of normal tissues including heart, placenta, and activated spleens (4). The wide expression of PD-L1 and PD-L2 and the inhibitor effects on PD-1 ligation indicate that PD-1 might be involved in the regulation of peripheral tolerance and may help prevent autoimmune diseases (2).

The human PD-1 gene encodes a 288 amino acid (aa) protein with a putative 20 aa signal peptide, a 148 aa extracellular region with one immunoglobulin-like V-type domain, a 24 aa transmembrane domain, and a 95 aa cytoplasmic region. The cytoplasmic tail contains two tyrosine residues that form the immuno-receptor
tyrosine-based inhibitory motif (ITIM) and immunoreceptor tyrosine-based switch motif (ITSM) that are important in mediating PD-1 signaling. Mouse and human PD-1 share approximately 60% aa sequence identity (4).

References
  1. Ishida, Y. et al. (1992) EMBO J. 11:3887.
  2. Nishimura, H. and T. Honjo (2001) Trends in Immunol. 22:265.
  3. Latchman, Y. et al. (2001) Nature Immun. 2:261.
  4. Carreno, B.M. and M. Collins (2002) Annu. Rev. Immunol. 20:29.
Long Name
Programmed Death-1
Entrez Gene IDs
5133 (Human); 18566 (Mouse); 301626 (Rat); 100533201 (Porcine); 486213 (Canine); 102123659 (Cynomolgus Monkey)
Alternate Names
CD279 antigen; CD279; hPD-1; PD1; PD-1; PD1hPD-l; PDCD1; programmed cell death 1; programmed cell death protein 1; Protein PD-1; SLEB2

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Citations for Human PD-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

34 Citations: Showing 1 - 10
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  1. Modulation of immune checkpoint regulators in interferon ? induced urothelial carcinoma and activated T-lymphocyte cells by cytostatics
    Authors: H�nze, J;Schulte-Herbr�ggen, J;Hofmann, R;Hegele, A;
    Genes and immunity
    Species: Human
    Sample Types: Protein
    Applications: Western Blot
  2. Exosomes in malignant pleural effusion from lung cancer patients impaired the cytotoxicity of double-negative T cells
    Authors: J Wu, R Zhu, Z Wang, X Chen, T Xu, Y Liu, M Song, J Jiang, Q Ma, Z Chen, Y Liu, X Wang, M Zhang, M Huang, N Ji
    Translational Oncology, 2022-10-14;27(0):101564.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Escherichia coli-specific CXCL13-producing TFH are associated with clinical efficacy of neoadjuvant PD-1 blockade against muscle-invasive bladder cancer
    Authors: AG Goubet, L Lordello, C Alves Cost, I Peguillet, M Gazzano, MD Mbogning-F, C Thelemaque, C Lebacle, C Thibault, F Audenet, G Pignot, G Gravis, C Helissey, L Campedel, M Roupret, E Xylinas, I Ouzaid, A Dubuisson, M Mazzenga, C Flament, P Ly, V Marty, N Signolle, A Sauvat, T Sbarrato, M Filahi, C Davin, G Haddad, J Bou Khalil, C Bleriot, FX Danlos, G Dunsmore, K Mulder, A Silvin, T Raoult, B Archambaud, S Belhechmi, I Gomperts B, N Cayet, M Moya-Nilge, A Mallet, R Daillere, E Rouleau, C Radulescu, Y Allory, J Fieschi, M Rouanne, F Ginhoux, G Le Teuff, L Derosa, A Marabelle, J VAN Dorp, N VAN Dijk, MS van der He, B Besse, F Andre, M Merad, G Kroemer, JY Scoazec, L Zitvogel, Y Loriot
    Cancer Discovery, 2022-10-05;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  4. Ex vivo-expanded human CD19+TIM-1+ regulatory B cells suppress immune responses in vivo and are dependent upon the TIM-1/STAT3 axis
    Authors: S Shankar, J Stolp, SC Juvet, J Beckett, PS Macklin, F Issa, J Hester, KJ Wood
    Nature Communications, 2022-06-03;13(1):3121.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  5. Additive Intralesional Interleukin-2 Improves Progression-Free Survival in a Distinct Subgroup of Melanoma Patients with Prior Progression under Immunotherapy
    Authors: D Rafei-Sham, S Lehr, M Behrens, F Meiss
    Cancers, 2022-01-21;14(3):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  6. The role of the immunoescape in colorectal cancer liver metastasis
    Authors: C Takasu, S Yamashita, Y Morine, K Yoshikawa, T Tokunaga, M Nishi, H Kashihara, T Yoshimoto, M Shimada
    PLoS ONE, 2021-11-19;16(11):e0259940.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  7. Regulation of PD-L1 expression in K-ras-driven cancers through ROS-mediated FGFR1 signaling
    Authors: C Glorieux, X Xia, YQ He, Y Hu, K Cremer, A Robert, J Liu, F Wang, J Ling, PJ Chiao, P Huang
    Redox Biology, 2020-11-03;38(0):101780.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. Impact of sidedness of colorectal cancer on tumor immunity
    Authors: C Takasu, M Nishi, K Yoshikawa, T Tokunaga, H Kashihara, T Yoshimoto, M Shimada
    PLoS ONE, 2020-10-12;15(10):e0240408.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  9. The innate immune effector ISG12a promotes cancer immunity by suppressing the canonical Wnt/&beta-catenin signaling pathway
    Authors: R Deng, C Zuo, Y Li, B Xue, Z Xun, Y Guo, X Wang, Y Xu, R Tian, S Chen, Q Liu, J Chen, J Wang, X Huang, H Li, M Guo, X Wang, M Yang, Z Wu, J Wang, J Ma, J Hu, G Li, S Tang, Z Tu, H Ji, H Zhu
    Cell. Mol. Immunol., 2020-09-22;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  10. Concordance of PD-1 and PD-L1 (B7-H1) in paired primary and metastatic clear cell renal cell carcinoma
    Authors: JE Eckel-Pass, TH Ho, DJ Serie, JC Cheville, R Houston Th, BA Costello, H Dong, ED Kwon, BC Leibovich, AS Parker
    Cancer Med, 2019-12-12;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  11. m6A mRNA demethylase FTO regulates melanoma tumorigenicity and response to anti-PD-1 blockade
    Authors: S Yang, J Wei, YH Cui, G Park, P Shah, Y Deng, AE Aplin, Z Lu, S Hwang, C He, YY He
    Nat Commun, 2019-06-25;10(1):2782.
    Species: Human
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC
  12. Mechanisms utilized by feline adipose-derived mesenchymal stem cells to inhibit T lymphocyte proliferation
    Authors: N Taechangam, SS Iyer, NJ Walker, B Arzi, DL Borjesson
    Stem Cell Res Ther, 2019-06-25;10(1):188.
    Species: Feline
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  13. Low levels of SIV-specific CD8+ T cells in germinal centers characterizes acute SIV infection
    Authors: S Li, JM Folkvord, KJ Kovacs, RK Wagstaff, G Mwakalundw, AK Rendahl, EG Rakasz, E Connick, PJ Skinner
    PLoS Pathog., 2019-03-21;15(3):e1007311.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Types: Whole Tissue
    Applications: IHC
  14. Tumor-derived exosomal HMGB1 promotes esophageal squamous cell carcinoma progression through inducing PD1+ TAM expansion
    Authors: B Li, TN Song, FR Wang, C Yin, Z Li, JP Lin, YQ Meng, HM Feng, T Jing
    Oncogenesis, 2019-02-22;8(3):17.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  15. Analysis of expression of the PD-1/PD-L1 immune checkpoint system and its prognostic impact in gastroenteropancreatic neuroendocrine tumors
    Authors: M Sampedro-N, A Serrano-So, M Adrados, JM Cameselle-, C Blanco-Car, JM Cabezas-Ag, R Martínez-H, E Martín-Pér, JL Muñoz de N, JÁ Díaz, R García-Cen, J Caneiro-Gó, I Abdulkader, R González-A, M Marazuela
    Sci Rep, 2018-12-13;8(1):17812.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  16. Tumor-derived exosomes induce PD1+ macrophage population in human gastric cancer that promotes disease progression
    Authors: F Wang, B Li, Y Wei, Y Zhao, L Wang, P Zhang, J Yang, W He, H Chen, Z Jiao, Y Li
    Oncogenesis, 2018-05-25;7(5):41.
    Species: Human
    Sample Types: Whole Cells, Whole Tissue
    Applications: Functional Assay, IHC
  17. Altered Ratio of T Follicular Helper Cells to T Follicular Regulatory Cells Correlates with Autoreactive Antibody Response in Simian Immunodeficiency Virus-Infected Rhesus Macaques
    Authors: W Fan, AJ Demers, Y Wan, Q Li
    J. Immunol., 2018-04-02;0(0):.
    Species: Primate
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  18. Associations of Simian Immunodeficiency Virus (SIV)-Specific Follicular CD8+T Cells with Other Follicular T Cells Suggest Complex Contributions to SIV Viremia Control
    Authors: MA Rahman, KM McKinnon, TS Karpova, DA Ball, DJ Venzon, W Fan, G Kang, Q Li, M Robert-Gur
    J. Immunol., 2018-03-05;0(0):.
    Species: Primate
    Sample Types: Whole Tissue
    Applications: IHC
  19. Blockade of Tumor-Expressed PD-1 promotes lung cancer growth
    Authors: S Du, N McCall, K Park, Q Guan, P Fontina, A Ertel, T Zhan, AP Dicker, B Lu
    Oncoimmunology, 2018-01-29;7(4):e1408747.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  20. Quantitative Multiplexed Imaging Analysis Reveals a Strong Association between Immunogen-Specific B Cell Responses and Tonsillar Germinal Center Immune Dynamics in Children after Influenza Vaccination
    Authors: D Amodio, N Cotugno, G Macchiarul, S Rocca, Y Dimopoulos, MR Castrucci, R De Vito, FM Tucci, AB McDermott, S Narpala, P Rossi, RA Koup, P Palma, C Petrovas
    J. Immunol., 2017-12-13;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  21. Increased expression of programmed cell death protein 1 on NK cells inhibits NK-cell-mediated anti-tumor function and indicates poor prognosis in digestive cancers
    Authors: Y Liu, Y Cheng, Y Xu, Z Wang, X Du, C Li, J Peng, L Gao, X Liang, C Ma
    Oncogene, 2017-07-10;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay, Neutralization
  22. Immune Cell Dynamics in Rhesus Macaques Infected with a Brazilian Strain of Zika Virus
    Authors: ELV Silveira, KA Rogers, S Gumber, P Amancha, P Xiao, SM Woollard, SN Byrareddy, MM Teixeira, F Villinger
    J. Immunol., 2017-06-30;0(0):.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Types: Whole Tissue
    Applications: IHC
  23. Particulate Array of Well-Ordered HIV Clade C Env Trimers Elicits Neutralizing Antibodies that Display a Unique V2 Cap Approach
    Authors: P Martinez-M, K Tran, J Guenaga, G Lindgren, M Àdori, Y Feng, GE Phad, N Vázquez Be, S Bale, J Ingale, V Dubrovskay, S O'Dell, L Pramanik, M Spångberg, M Corcoran, K Loré, JR Mascola, RT Wyatt, GB Karlsson H
    Immunity, 2017-05-16;46(5):804-817.e7.
    Species: Primate
    Sample Types: Whole Tissue
    Applications: IHC
  24. PD-L1, PD-L2 and PD-1 expression in metastatic melanoma: Correlation with tumor-infiltrating immune cells and clinical outcome
    Authors: Joseph M Obeid
    Oncoimmunology, 2016-09-20;5(11):e1235107.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  25. PD-1 Blockade with Pembrolizumab in Advanced Merkel-Cell Carcinoma
    Authors: PT Nghiem, S Bhatia, EJ Lipson, RR Kudchadkar, NJ Miller, L Annamalai, S Berry, EK Chartash, A Daud, SP Fling, PA Friedlande, HM Kluger, HE Kohrt, L Lundgren, K Margolin, A Mitchell, T Olencki, DM Pardoll, SA Reddy, EM Shantha, WH Sharfman, E Sharon, She
    N Engl J Med, 2016-04-19;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  26. Vaccine Induction of Lymph Node-Resident Simian Immunodeficiency Virus Env-Specific T Follicular Helper Cells in Rhesus Macaques.
    Authors: Vargas-Inchaustegui D, Demers A, Shaw J, Kang G, Ball D, Tuero I, Musich T, Mohanram V, Demberg T, Karpova T, Li Q, Robert-Guroff M
    J Immunol, 2016-01-15;196(4):1700-10.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Types: Whole Tissue
    Applications: IHC
  27. Plasmacytoid dendritic cells promote HIV-1-induced group 3 innate lymphoid cell depletion.
    Authors: Zhang Z, Cheng L, Zhao J, Li G, Zhang L, Chen W, Nie W, Reszka-Blanco N, Wang F, Su L
    J Clin Invest, 2015-08-24;125(9):3692-703.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  28. Soluble co-signaling molecules predict long-term graft outcome in kidney-transplanted patients.
    Authors: Melendreras S, Martinez-Camblor P, Menendez A, Bravo-Mendoza C, Gonzalez-Vidal A, Coto E, Diaz-Corte C, Ruiz-Ortega M, Lopez-Larrea C, Suarez-Alvarez B
    PLoS ONE, 2014-12-05;9(12):e113396.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Development
  29. Early lymphoid responses and germinal center formation correlate with lower viral load set points and better prognosis of simian immunodeficiency virus infection.
    Authors: Hong J, Amancha P, Rogers K, Courtney C, Havenar-Daughton C, Crotty S, Ansari A, Villinger F
    J Immunol, 2014-06-06;193(2):797-806.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Types: Whole Tissue
    Applications: IHC
  30. CD4 T follicular helper cell dynamics during SIV infection.
    J. Clin. Invest., 2012-08-27;122(9):3281-94.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Types: Whole Tissue
    Applications: IHC-P
  31. Modulation of T-cell activation by malignant melanoma initiating cells.
    Authors: Schatton T, Schutte U, Frank NY, Zhan Q, Hoerning A, Robles SC, Zhou J, Hodi FS, Spagnoli GC, Murphy GF, Frank MH
    Cancer Res., 2010-01-12;70(2):697-708.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  32. Early resolution of acute immune activation and induction of PD-1 in SIV-infected sooty mangabeys distinguishes nonpathogenic from pathogenic infection in rhesus macaques.
    Authors: Estes JD, Gordon SN, Zeng M, Chahroudi AM, Dunham RM, Staprans SI, Reilly CS, Silvestri G, Haase AT
    J. Immunol., 2008-05-15;180(10):6798-807.
    Species: Primate - Cercocebus torquatus (Sooty Mangabey), Primate - Macaca mulatta (Rhesus Macaque)
    Sample Types: Whole Tissue
    Applications: IHC-P
  33. Soluble PD-1 rescues the proliferative response of simian immunodeficiency virus-specific CD4 and CD8 T cells during chronic infection.
    Authors: Onlamoon N, Rogers K, Mayne AE, Pattanapanyasat K, Mori K, Villinger F, Ansari AA
    Immunology, 2008-02-05;124(2):277-93.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Types: Whole Cells
    Applications: Neutralization
  34. Aberrant regulation of synovial T cell activation by soluble costimulatory molecules in rheumatoid arthritis.
    Authors: Wan B, Nie H, Liu A, Feng G, He D, Xu R, Zhang Q, Dong C, Zhang JZ
    J. Immunol., 2006-12-15;177(12):8844-50.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Development

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Human PD-1 Antibody
By Anonymous on 11/23/2020
Application: ICC/IF Sample Tested: Melanoma tissue Species: Human
Immunocytochemistry/Immunofluorescence Human PD-1 Antibody AF1086
Human PD-1 Antibody
By Anonymous on 07/23/2018
Application: WB Sample Tested: 293T human embryonic kidney cell line Species: Human

293T cells were infected by lentivirus overexpression of control or PD-1 for 24 and 48 h. Total cell lysates were subjected to western blot. PVDF membrane were probed with 1 um/ml Human PD-1 Antibody (AF1086). A specific band was detected for PD-1 at approximately 43 kDa. This experiment was conducted under reducing conditions

Western Blot Human PD-1 Antibody AF1086
Human PD-1 Antibody
By Casey Keyes on 03/07/2018
Application: ELISA Sample Tested: Human recombinant antibody Species: Human
Human PD‑1 Antibody
By Anonymous on 11/24/2017
Application: Simple Western Sample Tested: 2120Ep human embryonal carcinoma cell line Species: Human
Human PD-1 Antibody
By Anonymous on 09/29/2017
Application: ELISA Sample Tested: Human recombinant test antibody Species: Human
Human PD-1 Antibody
By Anonymous on 03/14/2017
Application: Flow Sample Tested: Peripheral blood mononuclear cells (PBMCs) Species: Human

10^6 Human PBMCs were probed with 0.5 ug of Goat anti-Human PD-1 antibody (red), or Goat Isotype Control IgG (black), followed by PE-conjugated anti-Goat secondary antibody.

Flow Cytometry Human PD-1 Antibody AF1086