Human Pax6 PE-conjugated Antibody

Catalog #: IC8150P
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
IC8150P-100

Detection of Pax6 in Jurkat cells by Flow Cytometry.

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All Pax6 Antibodies

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Human Pax6 PE-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Pax6 in direct ELISAs. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) Pax1, rhPax2, rhPax3, rhPax4, rhPax5, and rhPax7 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human Pax6
Met1-Arg272
Accession # P26367

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration

Sample

Intracellular Staining by Flow Cytometry

10 µL/10⁶ cells

Jurkat human acute T cell leukemia cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry

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Detection of Pax6 in Jurkat cells by Flow Cytometry. Jurkat cells were stained with Sheep Anti-Human Pax6 PE‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC8150P, filled histogram) or isotype control antibody (Catalog # IC016P, open histogram). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: Pax6

Pax6 (paired box 6; also Oculorhombin) is a 48-50 kDa member of the paired homeobox family of transcription factors. It is expressed in developing optic vesicle, olfactory dopaminergic neurons, and pancreatic endocrine cells. Pax6 is a transactivating protein that interacts with MAF, CDX2 and SOX2. Human Pax6 is 422 amino acids (aa) in length. It contains an N-terminal paired box DNA-binding domain (aa 4-130), a Gly-rich central region (aa 131-209), a homeodomain (aa 213-269) and a C-terminal Pro/Ser/Thr-rich regulatory domain (aa 279-422). Phosphorylation of the C-terminal domain at Thr281/304/373 promotes Pax6 activity. Multiple splice forms of Pax6 exist. There are alternative start sites at Met137 and a position 34 aa upstream of the standard site. There is also a deletion of aa 22-26 and 37‑39, plus a 14 aa insertion after Gln47 that generates a C-terminal DNA binding site. Human and mouse Pax6 are absolutely identical in aa sequence.

Long Name

Paired Box Gene 6

Entrez Gene IDs

5080 (Human); 18508 (Mouse); 25509 (Rat)

Alternate Names

keratitis); MGC17209; Oculorhombin; paired box 6; paired box protein Pax-6; Pax6

Product Datasheets

Product Datasheet

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SDS

Related Research Areas

Citation for Human Pax6 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Glutamine-dependent signaling controls pluripotent stem cell fate
Authors: Vivian Lu, Irena J. Roy, Alejandro Torres, James H. Joly, Fasih M. Ahsan, Nicholas A. Graham et al.
Developmental Cell