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Human p21/CIP1/CDKN1A Antibody

Catalog #: MAB1047
3 Citations Datasheet / COA / SDS
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MAB1047
MAB1047-SP
Detection of Human p21/CIP1/CDKN1A by Western Blot.
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Citations (3)
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Human p21/CIP1/CDKN1A Antibody Summary

Species Reactivity
Human
Specificity
Detects human p21/CIP1/CDKN1A in direct ELISAs and Western blots.
Source
Monoclonal Mouse IgG2A Clone # 195720
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human p21/CIP1/CDKN1A
Ser2-Pro164
Accession # P38936
Formulation
Supplied as a 0.2 μm filtered solution in PBS. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Immunohistochemistry
8-25 µg/mL
See below
Immunoprecipitation
4 µg/500 µg cell lysate
MCF‑7 human breast cancer cell line treated with camptothecin (CPT), see our available Western blot detection antibodies
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human p21/CIP1/CDKN1A antibody by Western Blot. View Larger

Detection of Human p21/CIP1/CDKN1A by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line untreated (-) or treated (+) with 1 µM camptothecin (CPT) for 16 hours. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human p21/CIP1/CDKN1A Monoclonal Antibody (Catalog # MAB1047), followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for p21/CIP1/CDKN1A at approximately 21 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry p21/CIP1/CDKN1A antibody in Human Breast Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

p21/CIP1/CDKN1A in Human Breast Cancer Tissue. p21/CIP1/CDKN1A was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Mouse Anti-Human p21/ CIP1/CDKN1A Monoclonal Antibody (Catalog # MAB1047) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Intracellular Staining by Flow Cytometry Detection of p21/Cip1/CDKN1A antibody in MCF-7 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of p21/Cip1/CDKN1A in MCF‑7 Human Cell Line by Flow Cytometry. MCF-7 human breast cancer cell line either treated with 1 µM camptothecin (CPT) for 16 hours (filled histogram) or untreated (open histogram) was stained with Mouse Anti-Human p21/CIP1/CDKN1A Monoclonal Antibody (Catalog # MAB1047), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C, as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 6 months, -20 to -70 °C under sterile conditions after opening.

Background: p21/CIP1/CDKN1A

p21CIP1, also called CIP1 (CDK-interacting protein 1) and CDKN1A, is a 21 kDa Cyclin/Cyclin-dependent kinase (Cdk) inhibitor that blocks cell cycle progression from G1 to S phase in the cell cycle. Because p21 is a transcriptional target of the p53 tumor suppressor, p21 expression increases in cells that contain stabilized p53 due to genotoxic stress exposure.

Long Name
p21 Cyclin Dependent Kinase 4 Inhibitor 1A
Entrez Gene IDs
1026 (Human); 12575 (Mouse); 114851 (Rat)
Alternate Names
CAP20cyclin-dependent kinase inhibitor 1; CDK-interacting protein 1; CDKN1A; CDKN1melanoma differentiation associated protein 6; CIP1; CIP1WAF1CDK-interaction protein 1; cyclin-dependent kinase inhibitor 1A (p21, Cip1); MDA6; MDA-6; Melanoma differentiation-associated protein 6; p21; p21CIP1; p21Cip1/Waf1; PIC1; SDI1DNA synthesis inhibitor; wild-type p53-activated fragment 1

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Citations for Human p21/CIP1/CDKN1A Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Nitric oxide attenuated transforming growth factor-&beta induced myofibroblast differentiation of human keratocytes
    Authors: JH Park, M Kim, B Yim, CY Park
    Scientific Reports, 2021-04-14;11(1):8183.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Nitric oxide down-regulates polo-like kinase 1 through a proximal promoter cell cycle gene homology region.
    Authors: Zhang J, Wang S, Kern S, Cui X, Danner RL
    J. Biol. Chem., 2006-11-22;282(2):1003-9.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Tanshinone I induces apoptosis and protective autophagy in human glioblastoma cells via a reactive oxygen species‑dependent pathway
    Authors: Shangguan Jian, Liang Chen, Lian Minxue, Che Hongmin, Tang Ronghua, Fan Xiaoxuan et al.
    International Journal of Molecular Medicine

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