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Human Opticin Antibody

Catalog #: MAB3148 Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB3148
MAB3148-SP
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Opticin Antibody Summary

Species Reactivity
Human
Specificity
Detects human Opticin in direct ELISAs and Western blots. In direct ELIAs and Western blots, no cross-reactivity with recombinant mouse Opticin is observed.
Source
Monoclonal Rat IgG2A Clone # 361328
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Opticin
Ala20-Thr332
Accession # Q9UBM4
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human Opticin

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Opticin

Opticin (Oculoglycan) is a member of the small leucine-rich proteoglycan (SLRP) family with homology to class III extracellular matrix family members Epiphycan and Mimecan. It forms a 90 kDa noncovalent dimer that is a component of vitreous collagen fibrils in the eye and is also expressed in the retina, iris, ligaments and skin. Mature human Opticin shares 73% and 65% aa identity with mouse and rat, respectively. Mutations of Opticin have been associated with age-related macular degeneration.

Entrez Gene IDs
26254 (Human); 269120 (Mouse)
Alternate Names
oculoglycan; Oculogylcan; OPT; OPTC; Opticin

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