Human OLT-2/TARM1 Alexa Fluor® 647-conjugated Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Background: OLT-2/TARM1

T cell interacting, activating receptor on myeloid cells-1 (TARM-1), also known as OSCAR-like transcript-2 protein (OLT-2) is a type 1 membrane protein expressed by neutrophils, inflammatory monocytes, macrophages, and dendritic cells. Mature mouse OLT-2/TARM-1 contains a 242 aa long extracellular domain with two Ig-like C2 domains, a 21 aa long transmembrane domain and a 9 aa long cytoplasmic domain. The extracellular domain of human OLT-2/TARM-1 shares 48% and 49% amino acid (aa) identity with mouse and rat OLT-2/TARM-1 respectively. OLT-2/TARM-1 associates with the ITAM bearing adaptor FcR gamma, but not with DAP10 or DAP12.OLT-2/TARM1 expression is also up-regulated by bone marrow-derived macrophages and dendritic cells following stimulation with TLR agonists in vitro. OLT-2/TARM-1 receptor stimulation on macrophages and neutrophils co-stimulate the secretion of proinflammatory cytokines induced by TLR ligands, such as LPS. OLT-2/TARM-1 Fc fusion protein inhibits anti-CD3 induced CD4⁺ cell activation, suggesting that OLT-2/TARM-1 ectodomain interacts with an unidentified receptor on T cells to inhibit T cell activation (1).

Product Datasheets

Product Specific Notices

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

Related Research Areas

• Airway Inflammation and Remodeling
• T Cell Cytokine Signaling