Human Numb Antibody Summary
Thr356-Leu592
Accession # AAH68476
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human Numb by Western Blot. Western blot shows lysates of A549 human lung carcinoma cell line, HeLa human cervical epithelial carcinoma cell line, and U2OS human osteosarcoma cell line. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human Numb Monoclonal Antibody (Catalog # MAB4338) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Numb at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Numb in RT‑4 Human Cell Line. Numb was detected in immersion fixed RT‑4 human urinary bladder transitional cell papilloma cell line using Mouse Anti-Human Numb Monoclonal Antibody (Catalog # MAB4338) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Numb
Numb is a member of a family of cytoplasmic adaptor proteins termed CLASPS (Clathran-associated Sorting Proteins). These link transmembrane cargo proteins to the endocytic machinery. Numb, a conserved cell-fate determining factor, is normally associated with the basolateral surface of polarized cells. Movement towards the apical surface induces its phosphorylation, which precludes its entry into this region. Human Numb is 651 amino acids (aa) in length. It contains one PTB (aa 24‑172) and one PRR (aa 366‑413) domain. There is a potential for multiple alternate splice forms that involve all regions of the molecule. Over aa 356‑592, human Numb shares 88% and 90% aa identity with mouse and canine Numb, respectively.
Product Datasheets
Citation for Human Numb Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Numb regulates vesicular docking for homotypic fusion of early endosomes via membrane recruitment of Mon1b.
Authors: Shao X, Liu Y, Yu Q et al.
Cell Res.
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