Home / NTB-A/SLAMF6 / Human NTB-A/SLAMF6 Antibody

Human NTB-A/SLAMF6 Antibody

Catalog #: MAB1908
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB1908
MAB1908-SP
Product Details
Citations (1)
FAQs
Supplemental Products
Reviews
Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human NTB-A/SLAMF6 Antibody Summary

Species Reactivity
Human
Specificity
Detects human NTB‑A/SLAMF6 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human (rh) BLAME and rhCRACC is observed.
Source
Monoclonal Mouse IgG1 Clone # 292810
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human NTB-A/SLAMF6 Fc Chimera (Catalog # 1908-NT) under non-reducing conditions only

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Loading...
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: NTB-A/SLAMF6

NTB-A (NK-T-B-antigen), also known as Ly108 and SLAMF6, is a 60 kDa type I transmembrane glycoprotein that belongs to the SLAM subgroup of the CD2 family (1). Mature human NTB-A contains a 205 amino acid (aa) extracellular domain (ECD) with one Ig-like V-set and one Ig-like C2-set domain. It also contains a 21 aa transmembrane segment and an 84 aa cytoplasmic domain with two immunoreceptor tyrosine-based switch motifs (ITSMs) (2, 3). An alternately spliced isoform is truncated in the cytoplasmic domain and lacks the two ITSMs. Within the ECD, human NTB-A shares 48% aa sequence identity with mouse and rat NTB-A. The ECD of human NTB-A shares 19%‑34% aa sequence identity with comparable regions of human 2B4, BLAME, CD2F-10, CD84, CD229, CRACC, and SLAM. NTB-A is expressed on the surface of NK, T, and B lymphocytes as well as eosinophils (2, 4, 5). It interacts homophilically through weak associations between the Ig-V domains (2, 5‑7). NTB-A functions as an activating coreceptor on NK and T cells (2, 5, 6, 8). Tyrosine phosphorylation in the membrane proximal ITSM enables specific association with EAT-2, an interaction that is required for NTB-A mediated cytotoxicity of NK cells (9). Phosphorylation-dependent NTB-A association with SAP is required for full production of IFN-gamma by NK cells (5, 9). This interaction is independent of EAT-2 binding and appears to involve the membrane distal ITSM (5, 9). NTB-A deficient mice show weakened Th2 responses and elevated levels of neutrophil-derived inflammatory mediators (10). On B cells, NTB-A modulates immunoglobulin class switching and the balance between tolerance and autoimmunity (5, 11).

References
  1. Veillette, A. (2006) Immunol. Rev. 214:22.
  2. Bottino, C. et al. (2001) J. Exp. Med. 194:235.
  3. Fraser, C.C. et al. (2002) Immunogenetics 53:843.
  4. Munitz, A. et al. (2005) J. Immunol. 174:110.
  5. Valdez, P.A. et al. (2004) J. Biol. Chem. 279:18662.
  6. Flaig, R.M. et al. (2004) J. Immunol. 172:6524.
  7. Cao, E. et al. (2006) Immunity 25:559.
  8. Stark, S. and C. Watzl (2006) Int. Immunol. 18:241.
  9. Eissmann, P. and C. Watzl (2006) J. Immunol. 177:3170.
  10. Howie, D. et al. (2005) J. Immunol. 174:5931.
  11. Kumar, K.R. et al. (2006) Science 312:1665.
Long Name
NK-T-B Antigen/SLAM Family Member 6
Entrez Gene IDs
114836 (Human)
Alternate Names
Activating NK receptor; CD352 antigen; CD352; KALIFLJ50657; Ly108; NK-T-B-antigen; NTBA receptor; NTBA; NTB-A; NTB-AMGC104953; NTBAT- and B-cell antigen; SF2000; SLAM family member 6; SLAMF6

Product Datasheets

You must select a language.

x
Product Datasheet
COA
SDS

Citation for Human NTB-A/SLAMF6 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Transcriptional Regulation of the Endogenous Danger Signal Tenascin-C: A Novel Autocrine Loop in Inflammation
    Authors: Fui G. Goh, Anna M. Piccinini, Thomas Krausgruber, Irina A. Udalova, Kim S. Midwood
    The Journal of Immunology

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Human NTB-A/SLAMF6 Antibody

There are currently no reviews for this product. Be the first to review Human NTB-A/SLAMF6 Antibody and earn rewards!

Have you used Human NTB-A/SLAMF6 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review