Human Notch-3 PE-conjugated Antibody

Catalog #: FAB6870P Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
FAB6870P

Detection of Notch‑3 in BG01V Human Stem Cells by Flow Cytometry.

2 Images

All Notch-3 Antibodies

Product Details

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Notch-3 PE-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Notch-3 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) Notch-3 ICD (aa 2195-2321), rhNotch-1, rhNotch-1 ICD (aa 2251-2556), rhNotch-2, rhNotch-2 ICD (aa 2063-2413), rhNotch-4, rhNotch-4 ICD (aa 1778-2003), recombinant mouse Notch-3, or recombinant rat DLL1 is observed.

Source

Monoclonal Mouse IgG₁ Clone # 603532

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Notch-3
Ala40-Glu467
Accession # Q9UM47

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

View Larger

Detection of Notch‑3 in BG01V Human Stem Cells by Flow Cytometry. BG01V human embryonic stem cells were stained with Mouse Anti-Human Notch-3 PE-conjugated Monoclonal Antibody (Catalog # FAB6870P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry

View Larger

Detection of Notch‑3 in SUP‑T1 Human Cell Line by Flow Cytometry SUP‑T1 human T cell lymphoblastic lymphoma cell line was stained with Mouse Anti-Human Notch‑3 PE‑conjugated Monoclonal Antibody (Catalog # FAB6870P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: Notch-3

Human Notch-3 is a member of the Notch family of type I transmembrane glycoproteins involved in early-event developmental processes. The 2321 amino acid (aa) Notch-3 precursor contains a 1603 aa extracellular region with 34 EGF-like repeats. Repeats 1-11 of human Notch-3 are within the sequence used as an immunogen, and share 94% aa identity with mouse and rat Notch-3. Repeats 11 and 12 are critical for binding the ligands Jagged and Delta. Notch-3 is expressed in vascular smooth muscle, proliferating neuroepithelium, CD4^-CD8^- thymocytes, regulatory T cells and T-ALL leukemia cells. Mutations in the first 5 EGF repeats of Notch-3 in humans can cause CADASIL (cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy).

Entrez Gene IDs

4854 (Human); 18131 (Mouse)

Alternate Names

CADASIL; CASILneurogenic locus notch homolog protein 3; Notch (Drosophila) homolog 3; notch 3Notch homolog 3 (Drosophila); Notch homolog 3; Notch3; Notch-3