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Human NOD1 Antibody

Catalog #: MAB7090
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB7090
MAB7090-SP
Detection of Human NOD1 by Western Blot.
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Product Details
Citations (1)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human NOD1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human NOD1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human NOD2 is observed.
Source
Monoclonal Mouse IgG2B Clone # 626919
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human NOD1
Lys830-Phe953
Accession # Q9Y239
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunohistochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human NOD1 antibody by Western Blot. View Larger

Detection of Human NOD1 by Western Blot. Western blot shows lysates of HEK293T human embryonic kidney cell line either mock transfected or transfected with human NOD1 or human NOD2, HT-29 human colon adenocarcinoma cell line, and SW480 human colorectal adenocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human NOD1 Monoclonal Antibody (Catalog # MAB7090) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for NOD1 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Immunohistochemistry NOD1 antibody in Human Spleen by Immunohistochemistry (IHC-P). View Larger

NOD1 in Human Spleen. NOD1 was detected in immersion fixed paraffin-embedded sections of human spleen using Mouse Anti-Human NOD1 Monoclonal Antibody (Catalog # MAB7090) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in splenocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NOD1

NOD1, also called CARD4, is a 108 kDa intracellular pattern recognition receptor of the Nod-like receptor (NLR or Caterpiller) family. This innate immune receptor recognizes the muramyl peptide, diaminopimelic acid. The 953 amino acid (aa) NOD1 contains a caspase recognition domain (CARD), an NTPase domain and 10 leucine‑rich repeats (LRR). Splicing variants of 925 and 779 aa lack all or part of LRR 7-10 and do not respond to muramyl peptides. Polymorphisms of NOD1 are associated with inflammatory bowel disease. Within aa 830-953 (LRR 7-110), human NOD1 shares 82% and 84% aa identity with mouse and rat NOD1, respectively.

Long Name
Nucleotide-binding Oligomerization Domain-containing Protein 1
Entrez Gene IDs
10392 (Human); 107607 (Mouse); 500133 (Rat)
Alternate Names
CARD4; CARD4NLR family, CARD domain containing 1; caspase recruitment domain family, member 4; Caspase recruitment domain-containing protein 4; CLR7.1; NLRC1; NOD1; nucleotide-binding oligomerization domain containing 1; nucleotide-binding oligomerization domain, leucine rich repeat and CARD domaincontaining 1; nucleotide-binding oligomerization domain-containing protein 1

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Citation for Human NOD1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. HPV-mediated down-regulation of NOD1 inhibits apoptosis in cervical cancer
    Authors: X Liu, H Ma, L Fei, M Jiang, M Xia, L Bai, X Pi, S Chen, L Yu
    Infect. Agents Cancer, 2020-01-30;15(0):6.
    Species: Human
    Sample Types: Cell Culture Lysates, Whole Tissue
    Applications: IHC, Western Blot

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