Human NMNAT-1 Antibody Summary
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of NMNAT‑1 in Human Kidney. NMNAT‑1 was detected in immersion fixed paraffin-embedded sections of Human Kidney using Mouse Anti-Human NMNAT‑1 Monoclonal Antibody (Catalog # MAB5865) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell nuclei in convoluted tubules. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
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Preparation and Storage
Background: NMNAT-1
NMNAT-1 is expressed in the nuclei of all human tissues, with highest expression in skeletal muscle, heart, kidney, pancreas, and brain (1). The enzyme transfers adenylate from ATP to nicotinamide ribonucleotide or nicotinate ribonucleotide to generate NAD+ or deamido-NAD+, and is an essential enzyme for the production of nuclear NAD+ (2). Nuclear NAD+ is required by poly(ADP-ribose) polymerase 1 (PARP-1), which poly-ADP-ribosylates chromatin in response to DNA strand breaks. NMNAT-1 is known to interact with PARP-1, resulting in its activation, but this interaction with PARP-1 is prevented when NMNAT-1 is phosphorylated at Ser136 (3). Nuclear NAD+ levels are also important for the regulation of SIR2 histone deacetylases (4). A naturally occurring Ube4b/NMNAT-1 chimeric protein is directly involved in slowing the degeneration of injured neurons in mice (5). NMNAT activity is required for the activation of tiazofurin, a drug used to treat leukemia (6). Two other NMNAT enzymes are present in humans. NMNAT-2 is localized in the Golgi complex and cytoplasm, and NMNAT-3 is a mitochondrial enzyme (7).
- Emanuelli, M. et al. (2001) J. Biol.Chem. 276:406.
- Schweiger, M. et al. (2001) FEBS Lett. 492:95.
- Berger, F. et al. (2007) Proc. Natl. Acad. Sci. USA 104:3765.
- Revollo, J.R. et al. (2004) J. Biol. Chem. 279:50754.
- Mack, T.G. et al. (2001) Nature Neurosci. 4:1199.
- Boulton, S. et al. (1997) Br. J. Cancer 76:845.
- Berger, F. et al. (2005) J. Biol. Chem. 280:36334.
Product Datasheets
Citation for Human NMNAT-1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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NAD salvage pathway machinery expression in normal and glaucomatous retina and optic nerve
Authors: JR Tribble, A Hagström, K Jusseaume, E Lardner, RC Wong, G Stålhammar, PA Williams
Acta neuropathologica communications, 2023-01-22;11(1):18.
Species: Human
Sample Types: Whole Tissue
Applications: IHC
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