Human NLRP2/NALP2 Antibody

Catalog #: AF4684 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF4684
AF4684-SP

Detection of Human NLRP2/NALP2 by Western Blot.

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All NLRP2/NALP2 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human NLRP2/NALP2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human NLRP2/NALP2 in Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human NLRP2/NALP2
Met1-Thr208
Accession # Q9NX02

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human NLRP2/NALP2 by Western Blot. Western blot shows lysates of A549 human lung carcinoma cell line and LNCaP human prostate cancer cell line. PVDF membrane was probed with 1 µg/mL of Human NLRP2/NALP2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4684) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for NLRP2/NALP2 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NLRP2/NALP2

NALP2 (NAcht Leucine-rich repeat Protein 2, also known as NLRP2 and PAN1) is a cytosolic, 121 kDa member of the NLRP family of proteins. It is expressed in macrophages and exhibits divergent effects on inflammation. Via NF kappa B, it suppresses TNF-alpha production in response to LPS; via procaspase-1, it promotes proIL-1 beta cleavage and release. Human NALP2 is 1062 amino acids (aa) in length. It contains an N-terminal Pyrin domain (aa 9‑90), followed by a Nacht region and nine LRRs (aa 467‑1033). Four alternate isoforms exist. Three show deletions of aa’s 109‑132, 133‑154 and 847‑1062, respectively; a fourth shows a 38 aa substitution for aa’s 1‑903. No close rodent counterparts have been reported.

Long Name

NLR Family, Pyrin Domain Containing 2

Entrez Gene IDs

55655 (Human); 232827 (Mouse)

Alternate Names

CLR19.9; FLJ20510; NACHT, leucine rich repeat and PYD containing 2; NACHT, LRR and PYD domains-containing protein 2; NALP2; NALP2NACHT, LRR and PYD containing protein 2; NBS1; NBS1PYRIN-containing APAF1-like protein 2; NLR family, pyrin domain containing 2; NLRP2; nucleotide-binding oligomerization domain, leucine rich repeat and pyrin domaincontaining 2; PAN1; PAN1Nucleotide-binding site protein 1; PYPAF2; PYPAF2PYRIN domain and NACHT domain-containing protein 1