Human NKG2A/CD159a Antibody

Catalog #: MAB1059R Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB1059R-100
MAB1059R-SP

Detection of NKG2A/CD159a in Human PBMCs by Flow Cytometry.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human NKG2A/CD159a Antibody Summary

Species Reactivity

Human

Specificity

Recognizes the human NKG2A/CD94 heterodimer. It does not recognize the NKG2C/CD94 heterodimer or the CD94 homodimer.

Source

Recombinant Monoclonal Mouse IgG_2A Clone # 131411R

Purification

Protein A or G purified from cell culture supernatant

Immunogen

BaF3 mouse pro-B cell line transfected with human NKG2A and CD94

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of NKG2A/CD159a in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human NCAM-1/CD56 APC-conjugated Monoclonal Antibody (Catalog # FAB2408A) and either (A) Mouse Anti-Human NKG2A/CD159a Monoclonal Antibody (Catalog # MAB1059R) or (B) Mouse IgG2A Isotype Control (Catalog # MAB003) followed by Anti-Mouse IgG PE-conjugated secondary antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry

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Detection of NKG2A/CD159a in HEK293 Human Cell Line Transfected with Human NKG2A/CD159a and eGFP by Flow Cytometry. HEK293 Human embryonic kidney cell line transfected with either (A) Human NKG2A/CD159a, or (B) Human NKG2B/CD159b and eGFP was stained with Mouse Anti-Human NKG2A/CD159a Monoclonal Antibody (Catalog # MAB1059R) followed by APC-conjugated anti-Mouse IgG secondary antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB003). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NKG2A/CD159a

NKG2A, also known as CD159a, is a type II transmembrane receptor having a single extracellular lectin-like domain and a cytoplasmic ITIM motif. It associates with CD94 and is expressed on NK cells and some activated T cell populations. The NKG2A/CD94 complex delivers an inhibitory signal upon recognition of its ligand, HLA-E.

Long Name

Natural Killer G2A

Entrez Gene IDs

3821 (Human); 16641 (Mouse); 102121106 (Cynomolgus Monkey)

Alternate Names

CD159 antigen-like family member A; CD159a antigen; CD159a; C-lectin type II protein; killer cell lectin-like receptor subfamily C, member 1; Klrc1; MGC13374; natural killer cell lectin; natural killer group protein 2; NK cell receptor A; NKG2; NKG2-1/B activating NK receptor; NKG2A; NKG2-A; NKG2-A/B type II integral membrane protein; NKG2-A/B-activating NK receptor; NKG2-A/NKG2-B type II integral membrane protein; NKG2AMGC59791; NKG2-B