Human NGFR/TNFRSF16 APC-conjugated Antibody

Name: Human NGFR/TNFRSF16 APC-conjugated Antibody
Brand: R&D Systems
SKU: FAB367A
Rating: 4 (1 reviews)

Catalog #: FAB367A 1 Review Datasheet / COA / SDS

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FAB367A

Detection of NGF R/TNFRSF16 in SH‑SY5Y Human Cell Line by Flow Cytometry.

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All NGFR/TNFRSF16 Antibodies

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Human NGFR/TNFRSF16 APC-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human NGF R in direct ELISAs and Western blots. In direct ELISAs, no crossreactivity with recombinant human (rh) 4-1BB, rhCD27, rhCD40, rhBAFF R, rhCD30, rhDR3, rhDR6, rhEDAR, rhFas, rhHVEM, rhGITR, rhLTR B, recominant mouse (rm) NGF R, rhOPG, rmOX40, rhRANK, rhTAJ, rhTNF RI or rhTNF RII is observed.

Source

Monoclonal Mouse IgG₁ Clone # 74902

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human NGF R
Lys29-Asn250
Accession # P08138

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of NGF R/TNFRSF16 in SH‑SY5Y Human Cell Line by Flow Cytometry. SH-SY5Y human neuroblastoma cell line was stained with Mouse Anti-Human NGF R/TNFRSF16 APC-conjugated Monoclonal Antibody (Catalog # FAB367A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: NGFR/TNFRSF16

NGF R is a type I transmembrane protein that belongs to the tumor necrosis factor receptor family (1) and has been designated TNFRSF16. This receptor is also known as p75 NTR (neurotrophin receptor) because of its ability to bind at low affinity not only to NGF, but also other neurotrophins including brain-derived neurotrophic factor (BDNF), neurotrophin-3 and neurotrophin-4/5. NGF R is a 75 kDa protein that is expressed in neuronal axons, Schwann’s cells and perineural cells of peripheral nerves (1). Neural crest stem cells have been isolated based on their surface expression of NGF R (2, 3). In addition, neuroepithelial-derived NGF R positive cells have also been demonstrated to be able to differentiate into neurons, smooth muscle and Schwann cells in culture (4). NGF R has been used as a marker to identify mesenchymal precursors as well as hepatic stellate cells (5, 6).

References

Barker, P.A. et al. (1992) Mol. Cell Biochem. 110:1.
Stemple, D.L. et al. (1992) Cell 71:973.
Morrison, S.J. et al. (1999) Cell 96:737.
Mujtaba, T. et al. (1998) Dev. Biol. 200:1.
Campagnolo, L. et al. (2001) Biol. Reprod. 64:464.
Cassiman, D. et al. (2001) Hepatology 33:148.

Long Name

Nerve Growth Factor Receptor

Entrez Gene IDs

4804 (Human); 18053 (Mouse); 117089 (Rat)

Alternate Names

CD271 antigen; CD271; Gp80-LNGFR; member 16; nerve growth factor receptor; NGF R; NGF receptor; NGFR; p75 ICD; p75 NTR; TNFRSF16; TNFRSF16nerve growth factor receptor (TNFR superfamily, member 16); tumor necrosis factor receptor superfamily member 16