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Human NG2/MCSP Fluorescein-conjugated Antibody

Catalog #: FAB2585F
3 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB2585F
Detection of NG2/MCSP in G361 Human Cell Line by Flow Cytometry.
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Citations (3)
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Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human NG2/MCSP Fluorescein-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human NG2/MCSP in Western blots.
Source
Monoclonal Mouse IgG1 Clone # LHM-2
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human melanoma cells
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Fluorescein (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of NG2/MCSP antibody in G361 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of NG2/MCSP in G361 Human Cell Line by Flow Cytometry. G361 human melanoma cell line was stained with Mouse Anti-Human NG2/MCSP Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB2585F, filled histogram) or isotype control antibody (Catalog # IC002F, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: NG2/MCSP

NG2, also known as MCSP, is a chondroitin sulfate proteoglycan that has been used as a cell surface marker for melanoma (1) and glial precursor cells (2). NG2 also promotes epidermal stem cell patterned clustering (3).

References
  1. Kupsch, J.M. et al. (1995) Melanoma Res. 5:403.
  2. Stegmuller, J. et al. (2002) J. Neurocytol. 31:497.
  3. Legg, J. et al. (2003) Development 130:6049.
Long Name
Chondroitin Sulfate Proteoglycan NG2/Melanoma Associated Chondroitin Sulfate Proteoglycan
Entrez Gene IDs
1464 (Human); 121021 (Mouse); 81651 (Rat)
Alternate Names
AN2; chondroitin sulfate proteoglycan 4 (melanoma-associated); chondroitin sulfate proteoglycan 4; CSPG4; EC 3.6.3; HMW-MAA; MCSP; MCSPChondroitin sulfate proteoglycan NG2; MCSPG; MCSPGMelanoma chondroitin sulfate proteoglycan; MEL-CSPG; MSK16Melanoma-associated chondroitin sulfate proteoglycan; NG2; NG2EC 2.7.8

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Citations for Human NG2/MCSP Fluorescein-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Destabilization of NOXA mRNA as a common resistance mechanism to targeted therapies
    Authors: J Montero, C Gstalder, DJ Kim, D Sadowicz, W Miles, M Manos, JR Cidado, J Paul Secri, AE Tron, K Flaherty, F Stephen Ho, CH Yoon, A Letai, DE Fisher, R Haq
    Nat Commun, 2019-11-14;10(1):5157.
    Species: Human
    Sample Types: Whole Cells
    Applications: FACS
  2. Glucose transporter 1-positive endothelial cells in infantile hemangioma exhibit features of facultative stem cells.
    Authors: Huang L, Nakayama H, Klagsbrun M, Mulliken J, Bischoff J
    Stem Cells, 2015-01-01;33(1):133-45.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. The axonal repellent Slit2 inhibits pericyte migration: potential implications in angiogenesis.
    Authors: Guijarro-Munoz I, Cuesta AM, Alvarez-Cienfuegos A, Geng JG, Alvarez-Vallina L, Sanz L
    Exp. Cell Res., 2011-12-14;318(4):371-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Reviews for Human NG2/MCSP Fluorescein-conjugated Antibody

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Human NG2/MCSP Fluorescein-conjugated Antibody
By Christian Ostalecki on 06/07/2018
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Melanoma tissue Species: Human

Image: Skin (melanoma); MCSP (green), Cytokeratin 14 (red), Dapi (blue)

Immunocytochemistry/Immunofluorescence Human NG2/MCSP Fluorescein-conjugated Antibody FAB2585F