Human NFATC2 Antibody

Catalog #: MAB6499
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB6499
MAB6499-SP

Detection of Human NFATC2 by Western Blot.

1 Image

All NFATC2 Antibodies

Product Details

Citations (1)

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human NFATC2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human NFATC2 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human NFATC1 or C3 is observed.

Source

Monoclonal Mouse IgG_2B Clone # 639402

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human NFATC-2
His575-Pro679
Accession # Q13469

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

View Larger

Detection of Human NFATC2 by Western Blot. Western blot shows lysates of BJAB human Burkitt's lymphoma cell line, Jurkat human acute T cell leukemia cell line, and MOLT-4 human acute lymphoblastic leukemia cell line. PVDF Membrane was probed with 0.5 µg/mL of Human NFATC2 Monoclonal Antibody (Catalog # MAB6499) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for NFATC2 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NFATC2

NFATC2 (Nuclear factor of activated T cells C2; also NFAT1 or NFAT2P) is a 135 kDa member of the NFAT family of transcription factors. NFATC2 is found in T cells and mast cells where it regulates cytokine transcription, Th2 cell differentiation, and cell cycle entry. The transactivation function of NFATC2 is regulated by phosphorylation at Ser53, Ser56, Thr116, and Ser170. Human NFATC2 is 925 amino acids (aa) in length. It contains a calcineurin-binding site (aa 111-116), a transactivation domain (aa 119-199), two NLS (aa 251-253 and 664-666), an RHD that binds DNA (aa 392-574), and one NES (aa 904-913). An alternate splice form has a substitution of the C-terminal 18 amino acids. Human NFATC2 shares 96% aa identity with mouse and rat NFATC2.

Long Name

Nuclear factor of activated T-cells, cytoplasmic 2

Entrez Gene IDs

4773 (Human)

Alternate Names

NFAT pre-existing subunit; NFAT transcription complex, preexisting component; NFAT1; NFAT1nuclear factor of activated T-cells, cytoplasmic 2; NFATC2; NF-ATc2; NFATP; NF-ATp; NFATPnuclear factor of activated T-cells, preexisting component; nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2; preexisting nuclear factor of activated T-cells 2; T cell transcription factor NFAT1; T-cell transcription factor NFAT1

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human NFATC2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1