Human Neuropilin-1 APC-conjugated Antibody Summary
Phe22-Lys644
Accession # NP_001019799
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Neuropilin‑1 in HUVEC Human Cells by Flow Cytometry. HUVEC human umbilical vein endothelial cells were stained with Mouse Anti-Human Neuropilin-1 APC-conjugated Monoclonal Antibody (Catalog # FAB3870A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). View our protocol for Staining Membrane-associated Proteins.
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Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Neuropilin-1
Neuropilin-1 (Npn-1, previously neuropilin; also CD304) is a 130-140 kDa type I transmembrane (TM) glycoprotein that regulates axon guidance and angiogenesis (1-4). The full-length 923 amino acid (aa) human Npn-1 contains a 623 aa extracellular domain (ECD) that shows 92-95% aa identity with mouse, rat, bovine and canine
Npn-1 (3, 4). The ECD contains two N-terminal CUB domains (termed a1a2), two domains with homology to coagulation factors V and VIII (b1b2) and a MAM (meprin) domain (c). C-terminally divergent splice variants with 704, 644, 609, and 551 aa lack the MAM and TM domains and are demonstrated or presumed to be soluble antagonists (1, 5-7). A 906 aa form lacks a TM segment, but secretion has not been found (8). The sema domains of Class III secreted semaphorins such as Sema3A bind Npn-1 a1a2 (9). Heparin, the heparin-binding forms of VEGF (VEGF165, VEGF-B and VEGF-E), PlGF (PlGF2), and the C-terminus of Sema3 bind the b1b2 region (9, 10). Npn-1 and Npn-2 share 48% aa identity within the ECD and can form homo- and hetero-oligomers via interaction of their MAM domains (1). Neuropilins show partially overlapping expression in neuronal and endothelial cells during development (1, 2). Both neuropilins act as co‑receptors with plexins, mainly plexin A3 and A4, to bind class III semaphorins that mediate axon repulsion (11). However, only Npn-1 binds Sema3A, and only Npn-2 binds Sema3F (1). Both are co‑receptors with VEGF R2 (also called KDR or Flk-1) for VEGF165 binding (1). Sema3A signaling can be blocked by VEGF165, which has higher affinity for Npn-1 (12). Npn-1 is preferentially expressed in arteries during development or those undergoing remodeling (1, 2). Npn-1 is also expressed on dendritic cells and mediates DC-induced T cell proliferation (13).
- Bielenberg, D.R. et al. (2006) Exp. Cell Res. 312:584.
- Gu, C. et al. (2003) Dev. Cell 5:45.
- He, Z. and M. Tessier-Lavigne (1997) Cell 90:739.
- Soker, S. et al. (1998) Cell 92:735.
- Gagnon, M.L. et al. (2000) Proc. Natl. Acad. Sci. USA 97:2573.
- Cackowski, F.C. et al. (2004) Genomics 84:82.
- Rossignol, M. et al. (2000) Genomics 70:211.
- Tao, Q. et al. (2003) Angiogenesis 6:39.
- Gu, C. et al. (2002) J. Biol. Chem. 277:18069.
- Mamluk, R. et al. (2002) J. Biol. Chem. 277:24818.
- Yaron, A. et al. (2005) Neuron 45:513.
- Narazaki, M. and G. Tosato (2006) Blood 107:3892.
- Tordjman, R. et al. (2002) Nat. Immunol. 3477.
Product Datasheets
Citations for Human Neuropilin-1 APC-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 6
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Neuropilin-1 modulates the 3D invasive properties of glioblastoma stem-like cells
Authors: Mathilde Kerhervé, Sara Rosińska, Kilian Trillet, Alya Zeinaty, Magalie Feyeux, Steven Nedellec et al.
Frontiers in Cell and Developmental Biology
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Neuropilin-1 cooperates with PD-1 in CD8+ T cells predicting outcomes in melanoma patients treated with anti-PD1
Authors: Julien Rossignol, Zakia Belaid, Guillemette Fouquet, Flavia Guillem, Rachel Rignault, Pierre Milpied et al.
iScience
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Neuropilin-1 is upregulated in the adaptive response of prostate tumors to androgen-targeted therapies and is prognostic of metastatic progression and patient mortality
Authors: BW Tse, M Volpert, E Ratther, N Stylianou, M Nouri, K McGowan, ML Lehman, SJ McPherson, M Roshan-Mon, MS Butler, J Caradec, CY Gregory-Ev, J McGovern, R Das, M Takhar, N Erho, M Alshalafa, E Davicioni, EM Schaeffer, RB Jenkins, AE Ross, RJ Karnes, RB Den, L Fazli, PA Gregory, ME Gleave, ED Williams, PS Rennie, R Buttyan, JH Gunter, LA Selth, PJ Russell, CC Nelson, BG Hollier
Oncogene, 2017-01-16;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Neuropilin-1 expression characterizes T follicular helper (Tfh) cells activated during B cell differentiation in human secondary lymphoid organs.
Authors: Renand A, Milpied P, Rossignol J, Bruneau J, Lemonnier F, Dussiot M, Coulon S, Hermine O
PLoS ONE, 2013-12-30;8(12):e85589.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Expansive generation of functional airway epithelium from human embryonic stem cells.
Authors: McIntyre B, Alev C, Mechael R, Salci K, Lee J, Fiebig-Comyn A, Guezguez B, Wu Y, Sheng G, Bhatia M
Stem Cells Transl Med, 2013-12-03;3(1):7-17.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Neuropilin-1 is over-expressed in claudin-low breast cancer and promotes tumor progression through acquisition of stem cell characteristics and RAS/MAPK pathway activation
Authors: Yu Hin Tang, Anja Rockstroh, Kamil A. Sokolowski, Layla-Rose Lynam, Melanie Lehman, Erik W. Thompson et al.
Breast Cancer Research
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