Human Nestin Fluorescein-conjugated Antibody Summary
Accession # P48681
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Nestin in NTera‑2 Human Cell Line by Flow Cytometry. NTera-2 human testicular embryonic carcinoma cell line was stained with Mouse Anti-Human Nestin Fluorescein-conjugated Monoclonal Antibody (Catalog # IC1259F, filled histogram) or isotype control antibody (Catalog # IC002F, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
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Preparation and Storage
Background: Nestin
Nestin is a class VI intermediate filament protein that is expressed in stem cells of the central nervous system (CNS) but not in mature CNS cells. Nestin expression is used extensively as a marker for CNS stem cells in the developing nervous system and in vitro cultured cells. Its transient expression is a critical step in both the neural differentiation pathway and that of CNS glia. Nestin is also expressed in non-neural stem cell populations, such as pancreatic islet progenitors and hematopoietic progenitors. Full-length human Nestin shares 51% amino acid sequence identity to mouse Nestin.
Product Datasheets
Citations for Human Nestin Fluorescein-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 6
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Neural stem cell transplantation in patients with progressive multiple sclerosis: an open-label, phase 1 study
Authors: A Genchi, E Brambilla, F Sangalli, M Radaelli, M Bacigalupp, R Furlan, A Andolfo, D Drago, C Magagnotti, GM Scotti, R Greco, P Vezzulli, L Ottoboni, M Bonopane, D Capilupo, F Ruffini, D Belotti, B Cabiati, S Cesana, G Matera, L Leocani, V Martinelli, L Moiola, L Vago, P Panina-Bor, A Falini, F Ciceri, A Uglietti, MP Sormani, G Comi, MA Battaglia, MA Rocca, L Storelli, E Pagani, G Gaipa, G Martino
Nature Medicine, 2023-01-09;29(1):75-85.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Physiologic expansion of human heart-derived cells enhances therapeutic repair of injured myocardium
Authors: S Mount, P Kanda, S Parent, S Khan, C Michie, L Davila, V Chan, RA Davies, H Haddad, D Courtman, DJ Stewart, DR Davis
Stem Cell Res Ther, 2019-11-04;10(1):316.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Expression of a SOX1 overlapping transcript in neural differentiation and cancer models
Authors: Azaz Ahmad, Stephanie Strohbuecker, Cristina Tufarelli, Virginie Sottile
Cellular and Molecular Life Sciences
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Non integrative strategy decreases chromosome instability and improves endogenous pluripotency genes reactivation in porcine induced pluripotent-like stem cells
Authors: Annabelle Congras
Sci Rep, 2016-06-01;6(0):27059.
Species: Porcine
Sample Types: Whole Cells
Applications: Flow Cytometry -
Modeling Smith-Lemli-Opitz syndrome with induced pluripotent stem cells reveals a causal role for Wnt/?-catenin defects in neuronal cholesterol synthesis phenotypes
Nat. Med., 2016-03-21;22(4):388-96.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Arsenic trioxide-mediated Notch pathway inhibition depletes the cancer stem-like cell population in gliomas.
Authors: Zhen Y, Zhao S, Li Q
Cancer Lett., 2009-12-04;292(1):64-72.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
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We wanted to use this antibody to study expression of our fetal-derived neural stem cell lines in flow cytometry. We were not able to determine an appropriate antibody dilution using positive and negative control cell lines because the negative control line stained positive at all dilutions.
