Human Nestin Alexa Fluor® 594-conjugated Antibody

Catalog #: IC1259T Datasheet / COA / SDS

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IC1259T-100UG

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Human Nestin Alexa Fluor® 594-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Nestin.

Source

Monoclonal Mouse IgG1 Clone # 196908

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with human Nestin

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Label

Alexa Fluor 594 (Excitation= 590 nm, Emission= 617 nm)

Applications

Recommended Concentration

Sample

Intracellular Staining by Flow Cytometry

0.25-1 µg/10⁶ cells

A172 human glioblastoma cell line fixed with paraformaldehyde and permeabilized with saponin

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Nestin

Nestin is a class VI intermediate filament protein (1, 2) that is expressed in stem cells of the central nervous system (CNS) (3) but not in mature CNS cells (4). Nestin expression is used extensively as a marker for CNS stem cells in the developing nervous system and in vitro cultured cells (5-10). Its transient expression is a critical step in the neural differentiation pathway (2). Nestin is also expressed in non-neural stem cell populations, such as pancreatic islet progenitors (11-13) and hematopoietic progenitors (14).

References

Hockfield, S. and R.D. McKay (1985) J. Neurosci. 5:3310.
Lendahl, U. et al. (1990) Cell 60:585.
Frederiksen, K. and R.D. McKay (1988) J. Neurosci. 8:1144.
Tohyama, T. et. al. (1992) Lab. Invest. 66:303.
Uchida, N. et al. (2000) Proc. Natl. Acad.Sci. USA 97:14720.
Frederiksen, K. et al. (1988) Neuron 1:439.
Cattaneo, E. et al. (1990) Nature 347:762.
Reynolds, B.A. and S. Weiss (1992) Science 255:1707.
Rietze, R.L. et al. (2001) Nature 412:736.
Carpenter, M.K. et al. (2001) Exp. Neurol 172:383.
Zulewski, H. et al. (2001) Diabetes 50:521.
Lumelsky, N. et al. (2001) Science 292:1389.
Lechner, A. et al. (2002) Biochem.Biophys. Res. Commun. 293:670.
Shih, C.C. et al. (2001) Blood 98:2412.

Entrez Gene IDs

10763 (Human); 18008 (Mouse); 25491 (Rat)

Alternate Names

FLJ21841; NES; Nestin

Product Datasheets

Product Datasheet

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SDS

Product Specific Notices

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.