Human Nectin-1 Antibody

Name: Human Nectin-1 Antibody
Brand: R&D Systems
SKU: MAB2880
Rating: 5 (1 reviews)

Catalog #: MAB2880
1 Citation 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB2880
MAB2880-SP

Detection of Nectin‑1 in U937 Human Cell Line by Flow Cytometry.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Nectin-1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Nectin-1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Nectin-2, 3, 4, or recombinant mouse Nectin-1 is observed.

Source

Monoclonal Mouse IgG_2A Clone # 610835

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Nectin-1
Gln31-Thr334
Accession # Q15223

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Flow Cytometry

2.5 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of Nectin‑1 in U937 Human Cell Line by Flow Cytometry. U937 human histiocytic lymphoma cell line was stained with Human Nectin-1 Monoclonal Antibody (Catalog # MAB2880, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0101B).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Nectin-1

Nectin-1 (designated CD111), also called PRR-1 (poliovirus receptor-related protein 1) or HVEC (herpesvirus entry mediator C), is a widely expressed 110 kDa type I transmembrane glycoprotein important in formation of adherens junctions and synapses. It is a member of the nectin family within the Ig superfamily (1, 2). The Latin word necto means “to connect”, indicating the role of nectins in Ca²⁺-independent cell-cell adhesion (2). Nectin-1 forms homodimers in cis, followed by interactions in trans with Nectin-1, -3 or -4 (2). The 517 amino acid (aa) human Nectin-1 isoform 1 contains a 30 aa signal sequence, a 325 aa extracellular domain (ECD), a 21 aa transmembrane segment (TM), and a 141 aa cytoplasmic region. Nectin ECDs contain three Ig-like domains: an N-terminal V-type that mediates ligand binding and two C2-type (3). Nectin-1, like other nectins, has a splice form (isoform 2 or HigR, 458 aa) with alternate TM and cytoplasmic sequences. Another, isoform 3, is a 352 aa secreted protein (4). The common region of mature human Nectin-1 (aa 31-334) shares 93%, 94%, 96% and 96% aa identity with mouse, rat, bovine and porcine Nectin-1, respectively. Nectin-1 binds viral glycoprotein D to mediate herpesvirus (but not poxvirus) entry into vaginal mucosa, sensory neurons and fibroblasts (4 - 7). In forming adherens junctions and synapses, nectins 1 and 3 initiate cell-cell interactions, recruiting alpha _v beta ₃ integrin extracellularly and cadherins intracellularly through afadin and other junctional proteins (2, 8 - 11). These interactions organize the cytoskeleton, strengthen attachment to basement membrane and promote further cell-cell connections. Nectin-1 also recognizes CD96 on NK cells (12). Deficiency of Nectin-1 can result in cleft lip/palate ectodermal dysplasia (13). Nectin-1 downregulation in epithelial cancers, mediated in part by ectodomain shedding, may contribute to invasiveness (14).

References

Lopez, M. et al. (1995) Gene 155:261.
Takai, Y. et al. (2008) Nat. Rev. Mol. Cell Biol. 9:603.
Fabre, S. et al. (2002) J. Biol. Chem. 277:27006.
Lopez, M. et al. (2001) J. Virol. 75:5684.
Cocchi, F. et al. (1998) Proc. Natl. Acad. Sci. USA 95:15700.
Linehan, M. M. et al. (2004) J. Virol. 78:2530.
Simpson, S. A. et al. (2005) J. Neurovirol. 11:208.
Mizoguchi, A. et al. (2002) J. Cell Biol. 156:555.
Togashi, H. et al. (2006) J. Cell Biol. 174:141.
Tachibana, K. et al. (2000) J. Cell Biol. 150:1161.
Takai, Y. and H. Nakanishi (2003) J. Cell Science 116:17.
Seth, S. et al. (2007) Biochem. Biophys. Res. Commun. 364:959.
Suzuki, K. et al. (2000) Nat. Genet. 25:427.
Tanaka, Y. et al. (2002) Biochem. Biophys. Res. Commun. 299:472.

Long Name

Poliovirus Receptor Related 1

Entrez Gene IDs

5818 (Human); 58235 (Mouse); 102144697 (Cynomolgus Monkey)

Alternate Names

CD111 antigen; CD111; CLPED1ectodermal dysplasia 4 (Margarita Island type); ED4; Herpes virus entry mediator C; Herpesvirus entry mediator C; Herpesvirus Ig-like receptor; HIgRPVRR; HVEC; HVECpoliovirus receptor-like 1; MGC142031; Nectin1; Nectin-1; OFC7nectin 1; poliovirus receptor-related 1 (herpesvirus entry mediator C); poliovirus receptor-related protein 1; PRR; PRR1; PRR1MGC16207; PVRL1; PVRR1; PVRR1nectin-1; SK-12

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Citation for Human Nectin-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1